赵晶晶 郑福增 马俊福 马梦洋 郭嘉

【摘 要】目的：探讨雷公藤-鸡血藤药对治疗类风湿关节炎的作用机制。方法：使用中药系统药理学分析平台（TCMSP）筛选雷公藤-鸡血藤的活性成分，使用高通量基因表达数据库（GEO）、在线人类孟德尔遗传数据库（OMIM）、Genecards数据库筛选与类风湿关节炎相关的靶点，通过Cytoscape软件绘制“化合物-靶点”网络图。利用String数据库构建蛋白质相互作用（PPI）网络，利用R语言对靶点基因进行基因本体（GO）、京都基因与基因组百科全书（KEGG）生物通路富集分析。结果：筛选出木犀草素、山柰酚、雷公藤甲素、甘草查尔酮A等70种活性成分，信号转导与转录活化因子3、丝裂原活化蛋白激酶1、丝裂原活化蛋白激酶14等120个核心靶点，共同靶点主要涉及核受体的活动、乙型肝炎、前列腺癌、肿瘤坏死因子信号通路等1566个生物过程和152条信号通路。结论：对雷公藤-鸡血藤药对治疗类风湿关节炎的作用机制进行了初步探讨，为进一步阐述其作用机制奠定了基础。

【关键词】 关节炎，类风湿;网络药理学;雷公藤;鸡血藤;药对

【ABSTRACT】Objective：To explore the mechanism of the drug pair-Tripterygium Wilfordii and Caulis Spatholobi in the treatment of rheumatoid arthritis.Methods：TCMSP was used to screen the active components of Tripterygium Wilfordii and spatholobus Spatholobi.Databases GEO，OMIM and Genecards were used to screen the targets related to rheumatoid arthritis.The "compound target" network diagram was drawn by the software Cytoscape.The PPI network was constructed using the database String.The target genes were enriched in GO and KEGG by R language.Results：Seventy active components such as Luteolin，Kaempferol，Triptolide and Licochalcone A，and one hundred and twenty core targets，including signal transducer and activator of transcription 3，mitogen activated protein kinase 1 and mitogen activated protein kinase 14 were screened.The common targets mainly involved 1566 biological processes，such as nuclear receptor activity，hepatitis B，prostate cancer，tumor necrosis factor signaling pathway and 152 signal pathways.Conclusion：The mechanism of Tripterygium Wilfordii and Caulis Spatholobi in the treatment of rheumatoid arthritis was primarily discussed，which laid a foundation for further elaboration for its mechanism.

【Keywords】 arthritis，rheumatoid;networkpharmacology;Tripterygium Wilfordii;Caulis Spatholobi;drug pair

類风湿关节炎（rheumatoid arthritis，RA）是一种慢性炎症性疾病，特点是滑膜组织增生，一般会引起骨和软骨的破坏，最终导致关节畸形和残疾[1]。RA的病理改变是滑膜炎、血管翳形成并逐渐出现关节软骨和骨的破坏[2]。现代研究表明，中医药治疗RA能有效减少患者痛苦，提高预后生存质量，改善西药治疗的依赖性，减少对身体的伤害，具有明显的优势[3]。

雷公藤味苦、辛，性寒，有大毒，具有祛风湿、通经络、消肿止痛等功效。……