梁玉华 何花花 邓太平

[摘要]目的 运用网络药理学的方法预测牛黄治疗脑梗死的潜在靶点，探讨其调控网络及作用机制。方法 通过中药系统药理学数据库与分析平台（TCMSP）数据库和文献挖掘获取牛黄的主要活性成分，利用人类基因数据库GeneCards预测和筛选牛黄活性成分的作用靶点。采用String数据库和Cytoscape 3.7.1软件绘制蛋白相互作用（PPI）网络，借助DAVID6.8数据库进行潜在基因的基因本体论（GO）分析。结果 筛选得到牛黄5个活性成分，涉及3个作用靶点。GO分析结果显示，牛黄治疗脑梗死的生物过程主要涉及类固醇激素受体活性、核受体活性、转录因子活性、类固醇结合、维生素D受体结合、核受体结合、染色质DNA结合、ATP酶结合等。结论 牛黄治疗脑梗死是一个多成分、多靶点的复杂过程，主要通过调控NR3C2、PGR、RXRA這3个靶点，涉及核受体活性、转录因子活性、类固醇结合、维生素D受体结合、染色质DNA结合、ATP酶结合等生物过程而发挥干预脑梗死的作用。

[关键词]网络药理学;牛黄;脑梗死;潜在机制

[中图分类号] R282          [文献标识码] A          [文章编号] 1674-4721（2020）7（a）-0008-04

Study on the regulatory network and potential mechanism of Bezoar in the treatment of cerebral infarction

LIANG Yu-hua1   HE Hua-hua2   DENG Tai-ping3

1. Department of Pharmacy， Huizhou Municipal Central People′s Hospital， Guangdong Province， Huizhou   516001， China; 2. Department of Neurology， Huizhou Traditional Chinese Medicine Hospital， Guangdong Province， Huizhou   516001， China; 3. Department of Internal Medicine， Traditional Chinese Medicine Hospital of Boluo County in Huizhou City， Guangdong Province， Boluo   516100， China

[Abstract] Objective To predicit the potential target of Bezoar in the treatment of cerebral infarction via network pharmacology and to explore its regulatory network and mechanism of action. Methods The main active components of Bezoar were were obtained through traditional Chinese medicine systems pharmacology database and analysis platform （TCMSP） database and literature mining， and the target of the active ingredients of Bezoar was predicted and screened using the human gene database GeneCards. String database and Cytoscape 3.7.1 software were used to draw protein interaction （PPI） network， and gene ontology （GO） analysis of potential genes was performed with the application of DAVID6.8 database. Results A total of 5 active ingredients of Bezoar were obtained by screening， involving 3 targets. GO analysis results showed that the biological process of Bezoar in the treatment of cerebral infarction mainly involved steroid hormone receptor activity， nuclear receptor activity， transcription factor activity， steroid binding， vitamin D receptor binding， nuclear receptor binding， chromatin DNA binding， and ATPase binding， etc. Conclusion The treatment of cerebral infarction by Bezoar is a complex process with multiple components and multiple targets， mainly by regulating the three targets of NR3C2， PGR and RXRA， involving nuclear receptor activity， transcription factor activity， steroid binding， vitamin D receptor binding， chromatin DNA binding， ATPase binding and other biological processes to play a role in intervening cerebral infarction.

[Key words] Network pharmacology; Bezoar; Cerebral infarction; Potential mechanism

脑梗死是指各种原因引起脑部血液循环障碍，缺血、缺氧所致的局限性脑组织的缺血性坏死或软化，而出现相应的神经系统功能缺损，又称缺血性脑卒中[1]，是临床上常见的一种心脑血管疾病，具有发病急骤，致死、致残率高等特点[2]。脑梗死超过48 h病情逐渐恶化、加重，称为进展性脑梗死（progressive cerebral infarction，PCI）[3]。目前临床主要是采取常规药物治疗，包括抗凝、溶栓、抗血小板等，但对患者神经功能恢复难以取得较为理想的效果。……