张睿 王 湘 王玥琦 冯丽莉

摘要  目的：研究采用响应面法优化红景天抗氧化有效物质的提取工艺。方法：以乙醇浓度、提取时间、乙醇用量为主要影响因素，红景天苷提取率（%）、提取液体外DPPH清除率（%）和提取液体外总抗氧化能力为评价指标，采用3因素5水平响应面法对红景天抗氧化有效物质提取工艺进行优化。结果：Design-Expert软件优化得到的最佳提取工艺为，饮片加11倍体积46.60%乙醇溶液提取4.67 h，提取2次，合并提取液，浓缩，即得。该提取工艺下红景天苷提取率为1.12%，提取液DPPH清除率为89.13%，总抗氧化能力为1.39 mmol/L。结论：用响应面法优化得到的红景天抗氧化有效物质提取工艺简便易行，准确性和重现性好。

关键词  红景天;抗氧化;多因素;响应面法;红景天苷提取率;DPPH清除率;总抗氧化能力;提取工艺

Optimal Extraction of Herba Rhodiolae Antioxidant Active Ingredients by Multi-factors Response Surface Methodology

ZHANG Rui，WANG Xiang，WANG Yueqi，FENG Lili

（College of Basic Medicine，Beijing University of Chinese Medicine，Beijing 100029，China）

Abstract Objective： To optimize the extraction technology of Herba Rhodiolae antioxidant active ingredients by response surface methodology. Methods： Based on ethanol concentration，extraction time and dosage of ethanol as the main in uencing factors，the 3 factor 5 level response surface methodology was applied to optimize antioxidant active ingredients extraction of Herba Rhodiolae with evaluation indexes including extraction yield of Herba Rhodiolae （%），extracting liquid outside DPPH clearance （%） and extracting liquid outside total antioxidant activities（mmol/L）. Results： The optimal extraction process optimized by Design-Expert software was as follows：decoction peices adding 11 times of 46.60% ethanol solution and were extracted from for 4.67 h twice.The extracts were combined and concentrated.Under this extraction process，the extraction rate of Herba Rhodiolae is 1.12%，the DPPH removal rate of the extract was 89.13%，and the total antioxidant capacity was 1.39 mmol/L. Conclusion： The extraction process of Herba Rhodiolae antioxidants optimized by response surface method is simple，easy to implement，and has good accuracy and reproducibility.

Keywords  Herba Rhodiolae; Antioxidant active ingredients; Multi-factors，Response surface methodology; Extraction yield of Herba Rhodiolae; DPPH clearance; The total antioxidant activities by FRAP methods; Extraction technology

中图分类号：R284 文献标识码：A  doi： 10.3969/j.issn.1673-7202.2020.14.001

藏药红景天是景天科植物大花红景天的干燥根和根茎，始载于公元8世纪藏医药经典《四部医典》[1]，称其为“长生不老草”“九死还生草”，被誉为“高原人参”，具有扶正固本、理气养血等功效，《中华人民共和国药典》（2015版一部）谓其“味甘、苦，平。归肺、心经。功能益气活血、通脉平喘，用于气虚血瘀、胸痹心痛、中风偏瘫、倦怠气喘”[2]，是具有极高药用价值的珍贵药材。现代药理学研究表明，红景天具有抗肿瘤、抗抑郁、抗疲劳、抗缺氧和免疫调节等功效[3-5]。同时，红景天提取物具有卓越的抗氧化能力，被广泛应用于延缓机体衰老及防治氧化相關的老年性疾病[6-9]。另外，红景天服用安全，原卫生部2002年已批准其为可用于保健食品的中药品种[10]，是近年来国内炙手可热的保健中药，具有广阔的应用前景。……