石玥 刘雁峰 穆国华 王铁枫 姜晶 何波琳 乔婷 王佳楣

摘要 目的：探索骨碎补及其主要成分对胚泡着床障碍模型小鼠子宫内膜的影响。方法：实验动物分为7组：空白组、模型组、西药组I（补佳乐组）、西药组II（阿司匹林组）、骨碎补组、骨碎补总黄酮组、骨碎补柚皮苷组，以GnRHa+HMG+HCG方法造模，干预后检测各组相关指标的表达量。结果：骨碎补组及总黄酮组小鼠子宫内膜同源盒基因10（HOXA-10）mRNA表达明显增加（P<0.05）;骨碎补组、总黄酮组及柚皮苷组白血病抑制因子（LIF）表达明显增加（P<0.05）;骨碎补组整合素ανβ3表达明显增加（P<0.05），总黄酮组差异无统计学意义（P>0.05），但积分光密度值明显增加（P<0.05）。结论：骨碎补可能通过调节胚泡着床期子宫内膜HOXA 10、LIF、整合素ανβ3的表达，有利于胚泡对子宫内膜的黏附，从而改善子宫内膜容受性。总黄酮作为骨碎补药材的主要活性成分，亦可改善子宫内膜容受性。

关键词 骨碎补;总黄酮;柚皮苷;子宫内膜容受性;同源盒基因A10;白血病抑制因子;整合素ανβ3

Effect of Rhizoma Drynariae and Its Main Component on HOXA-10 mRNA，LIF and Integrin ανβ3 Expression in Mice Endometrium

SHI Yue1，LIU Yanfeng2，MU Guohua2，WANG Tiefeng2，JIANG Jing1，HE Bolin1，QIAO Ting1，WANG Jiamei1

（1 Haidian Maternal & Child Health Hospital，Beijing 100080，China; 2 Dongzhimen Hospital，Beijing University of Chinese Medicine，Beijing 100010，China）

Abstract Objective：To observe effects of rhizoma drynariae and its main components on endometrium of mice with blastocyst implantation disorder.Methods：Divide the experimental animals into 7 groups：a blank group，a model group，a western medicine treated group 1 （progynova group），a western medicine treated group 2 （aspirin group），a rhizoma drynariae group，an osteopractic total flavone group and a rhizoma drynariae naringin group.Mice model with GnRHa+HMG+HCG was established.We administrated drugs and tested the expression amount of each index in mice endometrium.Results：Expression of HOXA-10 mRNA in mice endometrium of rhizoma drynariae group and total flavone group increased significantly （P<0.05）.Rhizoma drynariae group，total flavone group and naringin group had increased LIF expression in mice endometrium （P<0.05）.Rhizoma drynariae group had an increased integrin ανβ3 expression in mice endometrium （P<0.05），there was no significant difference in total flavone group （P>0.05），but the integral optical density was significantly increased （P<0.05）.Conclusion：Rhizoma drynariae may regulate the expression of HOXA-10，LIF and integrin ανβ3 in endometrium during embryo implantation，which is conducive to the adhesion of blastocyst to endometrium，in order to improve endometrial receptivity.As the main active component of rhizoma drynariae，total flavonoids can also improve endometrial receptivity.

Keywords Rhizoma drynariael; Total flavone; Naringin; Endometrial receptivity; Homeobox A10; Leukaemia inhibitory factor; Integrin ανβ3

中圖分类号：R285.5;R271.41;R733.7文献标识码：Adoi：10.3969/j.issn.1673-7202.2020.24.007

骨碎补本是著名的骨伤科常用中药，因其补肾气，行血脉，益筋骨，为名老中医肖承悰教授治疗子宫内膜容受性低的经验方——二补助育汤的君药之一。子宫内膜容受性与体外受精-胚胎移植（IVF-ET）的成功与否具有较高相关性，通过中药来提高子宫内膜容受性逐渐成为被认可的治疗手段。针对二补助育汤开展的临床及实验研究取得了较好的研究结果[1-6]，但关于该方的生物活性及其影响子宫内膜容受性的确切作用机制仍在探索阶段，中药单体对药物机制探讨更有针对性。

骨碎补有补肾活血功用，骨碎补黄酮类化合物是其主要活性成分，柚皮苷为主要鉴定标准。故本实验选用骨碎补及其主要成分总黄酮、柚皮苷作为干预，以同源盒基因A10（Homobox Gene，HOX）、白血病抑制因子（Leukemia Inhibitory Factor，LIF）以及整合素ανβ3等影响子宫内膜容受性的公认指标开展研究，初步确定骨碎补影响子宫内膜容受性的主要活性成分及作用机制，为后续相关研究提供实验依据。……