林华坚 张梓豪 孟江 王淑美

中圖分类号 R283 文献标志码 A 文章编号 1001-0408（2020）10-1197-06

DOI 10.6039/j.issn.1001-0408.2020.10.08

摘 要 目的：评价干姜及其炮制品色差值与活性成分含量的相关性。方法：采用高效液相色谱法测定6种活性成分的含量，采用色彩色差计测定干姜及其炮制品的色差值[明度（L*）、红绿色轴分量（a*）、黄蓝色轴分量（b*）]。采用SPSS 24.0软件对色差值与活性成分含量进行相关性分析。结果：姜酮、6-姜酚、8-姜酚、6-姜烯酚、二乙酰氧基-6-姜二醇和10-姜酚检测质量浓度的线性范围分别为2.65～105.90、10.15～406.00、4.87～194.80、5.28～211.20、6.14～245.70、7.02～280.80 μg/mL（r均大于0.999）;定量限分别为7.46、13.68、14.37、16.62、17.03、17.99 ng，检测限分别为2.24、4.11、4.31、4.99、5.11、5.40 ng;精密度、稳定性、重复性试验的RSD均小于3%;平均加样回收率分别为101.34%、102.14%、101.22%，103.12%、103.74%、103.54%，103.06%、98.55%、99.43%，99.36%、103.51%、101.21%，100.85%、99.42%、99.60%，100.39%、97.69%、103.84%（RSD均小于3%，n=3）;含量分别为0～0.66、0.06～7.57、0.03～1.45、0.29～3.47、0.15～2.85、0.04～2.83 mg/g。色差值L*、b*与干姜不同炮制程度呈极显著负相关（P<0.01），a*与炮制程度呈正相关（P<0.05）;干姜炮制前后L*、b*与姜酮含量呈极显著负相关，与其余5种成分呈极显著正相关（P<0.01）;a*与姜酮呈极显著正相关（P<0.01），与其余5种成分无相关性（P>0.05）;干姜及其炮制品与姜酮含量呈极显著正相关，与其余5种成分含量呈极显著负相关（P<0.01）。结论：干姜及其炮制品的色差值与其活性成分含量相关，即随炮制程度的加重，其a*增加，L*、b*降低;姜酮含量升高，6-姜酚、8-姜酚、6-姜烯酚、二乙酰氧基-6-姜二醇、10-姜酚含量降低。

关键词 干姜;炮制品;高效液相色谱法;色彩色差计;活性成分;含量;色差值;相关性分析

Correlation Study of Color Difference Values and Active Constituent Contents in Crude and Processed Zingiber officinale

LIN Huajian，ZHANG Zihao，MENG Jiang，WANG Shumei（School of Traditional Chinese Medicine， Guangdong Pharmaceutical University/Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica， State Administration of Traditional Chinese Medicine/Engineering Technology Research Center for Chinese Materia Medica Quality of Guangdong Universities， Guangzhou 510006， China）

ABSTRACT   OBJECTIVE： To evaluate the correlation between color difference values and active constituent contents of crude and processed Zingiber officinale. METHODS： HPLC method was adopted to determint the content of 6 active constituents. The color difference values of crude and processed Z. officinale [lightness （L*）， red-green axis component （a*）， yellow-blue axis component （b*）] were determined by chromatic aberration meter. SPSS 24.0 software was adopted for the correlation analysis between color difference values and active constituent contents. RESULTS： The linear range of zingiberone， 6-gingerol， 8-gingerol， 6-shogaol， diacetoxy-6-gingerol and 10-gingerol were 2.65-105.90， 10.15-406.00， 4.87-194.80， 5.28-211.20， 6.14-245.70， 7.02-280.80 μg/mL （r>0.999）. The limits of quantification were 7.46，13.68，14.37，16.62，17.03，17.99 ng， and the limits of detection were 2.24，4.11，4.31，4.99，5.11，5.40 ng， respectively. RSDs of precision， stability， and repeatability tests were all lower than 3%. The average recovery rates were 101.34%， 102.14%， 101.22%; 103.12%， 103.74%， 103.54%; 103.06%， 98.55%， 99.43%; 99.36%， 103.51%， 101.21%; 100.85%， 99.42%， 99.60%; 100.39%， 97.69%， 103.84% （RSD were all lower than 3%，n=3）， respectively. The contents of them were 0-0.66， 0.06-7.57， 0.03-1.45， 0.29-3.47， 0.15-2.85， 0.04-2.83 mg/g， respectively. L* and b* values were negative correlated with the processing degree of Z. officinale significantly （P<0.01）， a* showed a significantly positive correlation with the processing degree （P<0.05）. L*and b* values showed a significantly negative correlation with the content of zingiberone before and after processing， but positively correlated with the other five components （P<0.01）. a* showed a significantly positive correlation with the content of zingiberone， but had no correlation with other five components （P>0.05）. The crude and processed Z. officinale were positive correlated with the content of zingiberone， negatively correlated with other five components （P<0.01）. CONCLUSIONS： There is a certain correlation between the color difference values of crude and processed Z. officinale and the contents of their active constituents. With the deepening of the processing， a* values is increased， L* values and b* values is decreased; the content of zingiberone increases， the contents of 6-gingerol， 8-gingerol， 6-shogaol， diacetoxy-6-gingerol， 10-gingerol reduce.

KEYWORDS   Crude Zingiber officinale; Processed product; HPLC; Chromatric aberration meter; Active constituent; Content; Color difference values; Correlation analysis

干姜为姜科植物姜（Zingiber officinale Rosc.）的干燥根茎，是一种药食同源的常见中药。该药性辛、热，具有温中散寒、回阳通脉、温肺化饮的功效[1]。干姜在临床上使用的炮制品种包括炮姜和姜炭，其中炮姜辛燥之性较干姜弱，但温经作用缓和持久，用于温经止痛[2];姜炭辛味消失，温经作用弱于炮姜，长于止血[2]。干姜及其炮制品主要含有姜辣素类活性成分，包括6-姜酚、8-姜酚、10-姜酚、6-姜烯酚、姜酮、二乙酰氧基-6-姜二醇等化合物[3-5]，具有抗氧化、解热抗炎、改善心血管系统等作用[6]。目前，干姜及其炮制品的质量控制主要包括6-姜酚的含量测定和传统的性状、颜色鉴别[1]。2……