于洪枫　牟洪波　戚大伟　俞莹

摘要：木材是我国可持续发展战略的重要部分，木材腐朽是破坏木材质量的主要途径之一，白腐菌是多种木腐菌中破坏木材细胞壁最为严重的。为抑制病原菌，提高木材利用率，利用低毒、低污染的甲苯胺蓝（TB）介导的光动力疗法对白腐菌抑制作用进行研究。通过牛津杯法测试不同浓度的TB（0.01、0.02、0.05 mg/mL）作用于白腐菌时，没有观察到抑菌作用。当采用低功率的630nm激光激发TB时，发现三组浓度光敏剂TB对白腐菌均起到抑制作用，随TB浓度增加抑菌率分别为69.67%、91.21%、99.44%。实验结果表明：甲苯胺蓝介导的光动力疗法对白腐菌具有较好的抑菌效果，且光敏剂浓度与其抑菌效果呈现正相关性，体现了良好的木材防腐效果。

关键词：光动力疗法;白腐菌;甲苯胺蓝;低功率激光光源

中图分类号：Q6-33;S763.15文献标识码：A文章编号：1006-8023（2019）02-0050-05

Effect of Toluidine Blue-mediated Photodynamic Therapy on White-rot Fungi

YU Hongfeng， MU Hongbo， QI Dawei， YU Ying

（College of Science， Northeast Forestry University， Harbin 150040）

Abstract：As an important part of Chinas sustainable development strategy， woods quality is mainly destroyed by wood decay. White-rot fungus is the most serious damage to wood cell wall in various wood rot fungi. In this paper， the inhibition of white-rot fungi was studied by using low toxicity and low pollution toluidine blue （TB） mediated photodynamic therapy to inhibit pathogenic bacteria， improve wood utilization. When the different concentrations （0.01， 0.02， 0.05 mg/mL） of TB were applied to white-rot fungi by the Oxford Cup method， no bacteriostatic effect was observed. However， when TB was excited by low-power 630nm laser， it was found that the three groups of photosensitizer TB inhibited white-rot fungi， and the inhibition rates with TB concentration were 69.67%， 91.21%， and 99.44%， respectively. The experimental results show that toluidine blue-mediated photodynamic therapy has a good antibacterial effect on white-rot fungi， and the concentration of photosensitizer is positively correlated with its antibacterial effect， which reflects the good anti-corrosion effect of wood.

Keywords：Photodynamic therapy; white-rot fungus; toluidine blue; low power laser light source

0引言

與世界多国相比，我国森林资源匮乏，木材防护水平相对落后，木材的用量又多，导致我国森林面积和质量都呈大幅度下降趋势，对现有森林资源的保护变得尤为重要 [1]。在影响木材质量的众多方面中，由木腐真菌引起的木材的腐朽问题最为常见。白腐菌是木腐菌中对木材腐朽比较严重的，它能够分泌木质素降解酶，具有较强的木质素降解能力 [2-3]。目前木材防腐主要以化学药剂为主，但这些包含砷、铬或铜的水溶性防腐剂对人类和自然环境都有较大的危害 [4]。因此，研究找到对人类和环境友好的木材防腐方法和木材防腐剂是十分必要的。……