孙秀超，买尔旦·玉苏甫，张　健，鲁彦坤，潘　佳，努尔威亚·努尔买买提，陶威熔，赛义德·谢卜利·艾哈迈德，魏媛媛

(新疆医科大学1基础医学院药理学教研室;2第一临床医学院；3国际教育学院；4基础医学院生理学教研室,乌鲁木齐　830011)

·基础医学研究·

石榴花多酚对离体大鼠胸主动脉环舒张作用的影响

孙秀超1，买尔旦·玉苏甫1，张健2，鲁彦坤2，潘佳2，努尔威亚·努尔买买提2，陶威熔2，赛义德·谢卜利·艾哈迈德3，魏媛媛4

(新疆医科大学1基础医学院药理学教研室;2第一临床医学院；3国际教育学院；4基础医学院生理学教研室,乌鲁木齐830011)

摘要:目的研究石榴花多酚(Pomegranate Flower Polyphenol，PFP)对离体大鼠胸主动脉环舒缩功能的影响及作用机制。方法采用离体血管环灌流法观察(100～900)mg/L PFP对大鼠胸主动脉环张力的影响，给予钾通道阻断剂和一氧化氮合酶抑制剂N-硝基-L-精氨酸甲酯(L-NAME)，测定大鼠血管组织中所含一氧化氮的含量。结果当PFP浓度为(700～900)mg/L时，与内皮去除组比较，内皮完整组PFP对大鼠胸主动脉环舒张作用更强，差异有统计学意义(P<0.01)。钾通道阻断剂格列本脲(Glib，10 μmol/L)、四乙基氯化铵(TEA，3mmol/L)、氯化钡(BaCl2，100 μmol/L)预处理均能抑制PFP对内皮去除大鼠胸主动脉环的舒张效应。PFP对无钙高钾液中Ca2+收缩曲线和无钙液中PE引起的内钙释放均有抑制作用。一氧化氮合酶抑制剂L-NAME预处理对PFP诱导的内皮完整大鼠胸主动脉环舒张作用具有抑制作用。结论石榴花多酚具有内皮依赖性和内皮非依赖性2种舒张血管作用。

关键词：石榴花多酚；血管舒张；一氧化氮；钾钙通道；血管环

中图分类号：R965

文献标识码：A

文章编号：1009-5551(2018)05-585-05

doi:10.3969/j.issn.1009-5551.2018.05.015

基金项目：国家自然科学基金(81360669)

作者简介：孙秀超(1990-)，男，在读硕士，研究方向：植物药药理。

通信作者：魏媛媛，女，博士，教授，硕士生导师，研究方向:植物药药理，E-mail：wei8923270@sina.com。

本文引用：孙秀超，买尔旦·玉苏甫，张健,等.石榴花多酚对离体大鼠胸主动脉环舒张作用的影响[J].新疆医科大学学报,2018,41(5):585-589.doi:10.3969/j.issn.1009-5551.2018.05.015

Diastoliceffectofpomegranateflowerpolyphenolonisolatedratthoracicaorticrings

SUNXiuchao1,MaierdanYusufu1,ZHANGJian2,LUYankun2,PANJia2,NuerweiyaNuermaimaiti2,TAOWeirong2,SyedShibliAhmed3,WEIYuanyuan4

(1DepartmentofPharmacology,SchoolofPre-clinicalMedicine,2the1stSchoolofClinicalMedicine,3InternationalEducationInstitute,4DepartmentofPhysiology,SchoolofPre-clinicalMedicine,XinjiangMedicalUniversity,Urumqi830011,China)

Abstract：ObjectiveTo investigate diastolic effect of Pomegranate Flower Polyphenol(PFP)on isolated rat thoracic aortic rings and its mechanism.MethodsThe tension of isolated thoracic aortic rings of rats in presence or absence of PFP(100～900mg/L)were observed by vascular ring perfusion method in vitro,then the mechanism were explored by application of Ca2+&K+channel blockers and nitric oxide synthase inhibitor L-NAME.And the content of nitric oxide in rat vascular tissue was determined.ResultsWhen the concentration of PFP was(700～900)mg/L,PFP in the intact endothelium group had a stronger effect on rat thoracic aortic rings dilation than that in the group with endothelial deprivation(P<0.01).Diastolic effect on denuded rat thoracic aortic rings induced by PFP could be inhibited by pretreatment of K+channel blockers:10 μmol/L Glib,3mmol/L TEA and 100 μmol/L BaCl2.PFP could move the calcium-constriction curve to the right in calcium-free high-potassium solution,and inhibit intracellular calcium releasing induced by PE in calcium-free solution.Pretreatment with nitric oxide synthase inhibitor L-NAME inhibited relaxation of thoracic aortic rings with intact endothelium induced by PFP.ConclusionPFP has endothelium-dependent and endothelium-independent vasodilation effects.

Keywords:Pomegranate flower polyphenols;vasodilation;nitric oxide;potassium/calcium channel;vessel ring

石榴花为石榴科(Punicaceae)石榴属(PunicL.)植物石……