地里夏提·库尔班

[摘要]目的：探讨微小RNA-21（miRNA-21）基因与转化生长因子-β1（transforming growth factor-β，TGF-β1）基因在正常皮膚组织和增生性瘢痕组织中的表达，并分析其相关性。方法：选取本院烧伤整形科收治的16例烧伤整形患者的增生性瘢痕组织及其临近的正常皮肤组织，提取组织中的总RNA，采用实时荧光定量PCR（realtime fluorescence quantitative PCR，RT-qPCR）技术分别检测增生性瘢痕组织和正常组织中miRNA-21与TGF-β1基因表达量，对检测结果进行分析比较，并行相关性分析。结果：增生性瘢痕组织中miRNA-21基因表达明显上调，差异具有统计学意义（P<0.05），其表达水平是正常皮肤组织的2.18倍；增生性瘢痕中TGF-β1的表达量多于正常皮肤组织，差异具有统计学意义（P<0.05），其表达水平是正常皮肤组织的2.31倍；增生性瘢痕组织中miRNA21基因表达量与TGF-β1基因表达量呈正相关（r=0.836，P<0.01）。结论：增生性瘢痕组织中miRNA-21和TGF-β1基因表达异常，可能与瘢痕组织形成相关。

[关键词]增生性瘢痕；微小RNA21；TGF-β1

[中图分类号]R619+.6 [文献标志码]A [文章编号]1008-6455（2017）10-0068-03

Abstract： Objective To investigate the expression of microRNA-21 （miRNA-21） gene and transforming growth factor-β（TGF-β1） gene in normal skin and hypertrophic scar tissue， and to analyze its correlation. Methods Collected from 16 hypertrophic scar tissues and adjacent normal skin tissues formed by burn and plastic surgery department in our hospital， total RNA was extracted from normal tissues and Hypertrophic scar tissue. The expressions of miRNA-21 and TGF-β1 gene in hypertrophic scar tissue and normal tissue were detected by RT-qPCR. The detection results were analyzed and compared， and the correlation analysis was carried out. Results The expression of miRNA-21 gene in hypertrophic scar tissue was significantly up-regulated， the difference was statistically significant（P<0.05）， its expression level was 2.18 times of normal skin tissue. The expression of TGF-β1 in hypertrophic scar was higher than that in normal skin tissue（P<0.05）. The expression level of TGF-β1 was 2.31 times higher than that of normal skin tissue. There was a positive correlation between the expression of miRNA-21 gene and the expression of TGF-β1 gene in hypertrophic scar tissue（r=0.836， P<0.01）. Conclusion The abnormal expression of miRNA-21 and TGF-β1 gene in hypertrophic scar tissue may be related to the formation of scar tissue.

Key words： hypertrophic scar； microRNA-21； TGF-β1

增生性瘢痕是伤口表皮愈合过程中组织过度增生而引起的病理性瘢痕，不具备正常皮肤结构和生理功能。严重创伤、烧伤及外科手术后常并发程度不等的增生性瘢痕，影响外貌美观，甚至可导致严重畸形及功能障碍，降低患者生活质量。具体发病机制尚未完全明确。但有研究表明，增生性瘢痕主要与成纤维细胞异常增殖及凋亡抑制、分泌和释放的正向及负向细胞因子调节机制的异常，以及细胞外基质胶原合成与降解的失平衡有关，包括整合素、TGF-β和miRNA的异常表达[1-2]。在增生性瘢痕与正常皮肤组织微小RNA芯片及基因芯片检测研究中发现，miRNA-21和TGF-β1在增生性瘢痕与正常皮肤组织中存在差异表达，可能与增生性瘢痕组织的发生、发展及演化密切相关[3]。但关于miRNA-21与TGF-β1基因在增生性瘢痕形成过程中是否起协同作用，目前相关研究报道尚罕见。且以往关于TGF-β1的研究多局限于免疫组化、原位杂交或半定量水平的研究。……