李冬梅++赵超艺++刘小飞

摘 要 以土田七的幼嫩根茎为外植体，通过对其芽启动诱导、继代增殖及生根诱导培养基的筛选，建立了土田七幼嫩根茎的离体快繁体系。结果表明：土田七幼嫩根茎的最佳芽诱导培养基为MS+6-BA 3.0 mg/L+NAA 0.10 mg/L+3%蔗糖+卡拉胶粉9.5 g/L+10%椰汁；继代增殖最佳的培养基为MS+6-BA 5.0 mg/L+NAA 0.10 mg/L+3%蔗糖+卡拉胶粉9.5 g/L+10%椰汁；生根最佳的培养基为MS+ NAA 0.10 mg/L+3%蔗糖+卡拉胶粉9.5 g/L+10%香蕉泥+活性碳1.0 g/L；组培苗移栽较合适的基质为进口泥炭∶红壤∶腐叶土混合的基质。

关键词 土田七 ；土田七属 ；根茎 ；繁殖

中图分类号 S682.36 文献标识码 A Doi：10.12008/j.issn.1009-2196.2017.10.011

Rapid Propagation of Stahlianthus involucratus via in Vitro Culture

of Young Rhizomes

LI Dongmei ZHAO Chaoyi LIU Xiaofei

（Environmental Horticulture Research Institute，

Guangdong Academy of Agricultural Sciences， Guangzhou， Guangdong 510640）

Abstrcat Young rhizomes of Stahlianthus involucratus were used as explants for tissue culture. Media for bud inducing， subculture and rooting were screened to establish a rapid propagation system based on young rhizomes of S. involucratus. The results showed that the optimum medium was MS+6-BA 3.0 mg/L+NAA 0.10 mg/L+3% sucrose+carrageenan powder 9.5 g/L+10% coconut water for inducing of adventitious buds， MS+6-BA 5.0 mg/L+NAA 0.10 mg/L+3% sucrose+carrageenan powder 9.5 g/L+10% coconut water for subculture， and MS+NAA 0.10 mg/L+3% sucrose+carrageenan powder 9.5 g/L+10% mashed banana + activated carbon 1.0 g/L for rooting. The optimum medium for transplanting of the rooted plantlets was the mixture of imported peat， red soil and humus soil.

Keywords Stahlianthus involucratus ； Stahlianthus ； rhizome ； propagation

土田七（Stahlianthus involucratus）屬于姜科（Zingiberaceae）土田七属（Stahlianthus）植物，产于广东、广西、云南、福建等地；印度和锡金也有分布[1-2]。土田七植株高15～30 cm，根茎外面棕褐色，内面棕黄色，芳香而有辛辣味，根末端膨大成球状；叶片披针形，叶面深绿色，叶被绿色或淡紫红色，叶柄较长；花序从根茎抽出，10～15朵花聚生于钟状的总苞内，总苞绿色；花白色，唇瓣中央具杏黄色斑，内被长柔毛；生于海拔800～900 m的林下、荒坡荫湿处；花期5～7月；观叶类，可作为林下地被和盆栽观叶植物[1，3-4]。土田七的根茎具药用价值，能活血散瘀，消肿止痛，主治跌打损伤，风湿骨痛[2，4]。

土田七可通过切分根茎分株的方式繁殖，但分株繁殖速度慢，且对母株的根茎也有损伤。黄赛等[5]研究表明，以土田七的茎尖为外植体诱导效果最好，30 d的增殖系数为4.16，但根茎为外植体表面灭菌较为困难，消毒时间长且容易污染，污染率达到34%。为了建立更优良的土田七的根茎组培快繁体系，本研究以土田七的幼……