王桂英 王耀帅 高建芸 苏方方 陈若暘

摘要：目的 观察不同温度艾灸热刺激对高脂血症大鼠血脂及背根神经节瞬时感受器电位香草酸受体1（TRPV1）mRNA表达的影响，验证艾灸调脂疗效与激活TRPV1相关。方法 采用高脂饲料喂养制作高脂血症大鼠模型。将60只SD大鼠随机分为正常组、模型组、38 ℃艾灸组和45 ℃艾灸组，每组15只。38 ℃艾灸组和45 ℃艾灸组均取“神阙”和双侧“足三里”，每穴艾灸10 min/次，隔日1次，连续4周。干预结束后取血，氧化酶法检测血清胆固醇、三酰甘油、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇含量；取背根神经节组织，实时荧光定量PCR检测TRPV1 mRNA表达。结果 与模型组比较，艾灸组血脂指标均得到不同程度的调整，45 ℃艾灸组差异有统计学意义（P<0.05，P<0.01），且45 ℃艾灸组与38 ℃艾灸组比较差异有统计学意义（P<0.01）；45 ℃艾灸组较其他3组背根神经节TRPV1 mRNA表达差异均有统计学意义（P<0.01）。结论 艾灸调脂疗效与艾灸激活TRPV1相关。

关键词：灸温；瞬时感受器电位香草酸受体1；血脂；大鼠

DOI：10.3969/j.issn.1005-5304.2017.11.011

中图分类号：R245 文献标识码：A 文章编号：1005-5304（2017）11-0044-04

Abstract： Objective To compare the effects of moxibustion at different temperatures on blood lipid and TRPV1 in dorsal root ganglion with hyperlipidemia rats； To verify the correlation between efficacy of adjusting fat of moxibustion with activating of TRPV1. Methods The rat model of hyperlipidemia was made by high fat diet. 60 SD mice were randomly divided into four groups： control group， model control group， the 38 ℃ moxibustion group and the 45 ℃ moxibustion group， with 15 mice in each group. Acupoints Shenque and Zusanli were chosen under moxibustion for 10 minutes each time， once another day， for 4 weeks， in the 38 ℃ moxibustion group and the 45 ℃ moxibustion group. Blood was taken after intervention， and blood TC， TG， LDL-C and HDL-C of the mice were detected by oxidase method； the dorsal root ganglion was taken to detect the expression of TRPV1 mRNA by real-time fluorescence quantitative PCR. Results Compared with model group， blood lipid indexes in moxibustion groups had different changes， with statistical significance compared with 45 ℃ moxibustion group （P<0.05， P<0.01）， and there was statistical significance between 38 ℃ moxibustion group and 45 ℃ moxibustion group （P<0.01）； there was statistical significance in TRPV1 mRNA of dorsal root ganglion among 45 ℃ moxibustion group and other three groups （P<0.01）. Conclusion The correlation between efficacy of adjusting fat of moxibustion with activating of TRPV1 has been confirmed.

Key words： moxibustion temperature； TRPV1； blood lipid； rats

基金項目：国家自然科学基金（81603695、81503648）

艾灸临床疗效有针刺治疗所不及的优势。《灵枢·官能》云：“针所不为，灸之所宜。”吴焕淦等[1]认为，以灸法临床有效的病症为载体，开展基于临床灸法作用机理研究，阐明艾灸作用特点及机理，有利于灸法研究的新发展。课题组前期研究发现，不同温度艾灸热刺激对急性高脂血症模型小鼠胆固醇调整有差异[2]，为了研究艾灸调整其他血脂组分的普遍意义及与瞬时感受器电位香草酸受体1（TRPV1）相关性，本实验仍以临床灸法的有效病种高脂血症为载体，选用慢性高脂血症模型大鼠，以不同温度（38 ℃和45 ℃）作为实验的干预条件，观察不同……