秦 枫，朱善元，成大荣，陈玉勇，左伟勇，王安平，王永娟，吴 双，洪伟鸣

（1. 江苏农牧科技职业学院 江苏省兽用生物制药高技术研究重点实验室，泰州 225300；2. 扬州大学兽医学院，扬州 225009）

三七皂苷S-6的溶血性及体外免疫活性研究

秦 枫1,2，朱善元1，成大荣1,2，陈玉勇1，左伟勇1，王安平1，王永娟1，吴 双1，洪伟鸣1

（1. 江苏农牧科技职业学院 江苏省兽用生物制药高技术研究重点实验室，泰州 225300；2. 扬州大学兽医学院，扬州 225009）

研究三七皂苷S-6 的溶血性及体外免疫活性。常规方法提取PNS，大孔树脂柱层析分离纯化得S-6。溶血试验测定红细胞溶血率。脾淋巴细胞毒性试验确定S-6 的安全剂量范围，体外脾淋巴细胞增殖反应和IL-2的诱生及含量测定检测S-6 的体外免疫活性。S-6 溶血性较小，2 mg/mL浓度以下无溶血性；S-6（0.1 μg/mL、1 μg/mL）显著促进了Con A诱导的小鼠脾淋巴细胞增殖反应，且与PNS和APS组相比，差异不显著；S-6（1 μg/mL）极显著地促进了LPS诱导的小鼠脾淋巴细胞增殖反应，且与PNS相比，S-6（1 μg/mL）差异显著，其余各个剂量与PNS和APS组相比，差异不显著；S-6（12.5 μg/mL、25 μg/mL、50 μg/mL）显著或极显著地提高了IL-2的含量，且与PNS和APS组相比，差异不显著。S-6 安全性好，体外具有较好的细胞免疫活性和诱生细胞因子的能力。

S-6；溶血性；体外免疫活性；脾淋巴细胞增殖反应；IL-2

Abstract:To investigate the hemolytic characteristics and biologic immunocompetence of S-6 in vitro, PNS was extracted using butyl alcohol and S-6 was obtained by separation and puri fi cation with D101 macroreticular resin. The in vitro hemolytic activity, range of safe dose and immunocompetence of S-6 were examined for parameters including hemolysis, inducible and content determination of IL-2, splenocyte toxicity and proliferation. The hemolytic activity of S-6 was lower than 2 mg/mL. S-6 significantly increased concanavalin A-stimulated splenocyte proliferation in vitro at 0.1 μg/mL. However, S-6 had no signi fi cant difference with PNS and APS at 1 μg/mL. S-6 signi fi cantly increased concanavalin lipopolysaccharide-stimulated splenocyte proliferation in vitro at 1 μg/mL and had signi fi cant difference with PNS. S-6 also increased the content of IL-2 at 12.5 μg/mL, 25 μg/mL and 50 μg/mL and had no signi fi cant difference with PNS and APS. In conclusion, S-6 had high security and increased cellular response and in vitro content of IL-2.

Key words:S-6; hemolytic characteristics; immunocompetence in vitro; splenocyte proliferation; IL-2

近年来，新城疫、禽流感、口蹄疫、猪蓝耳等畜禽疾病在各地频繁爆发，给我国畜牧养殖业带来重大损失。疫苗免疫是目前控制动物传染病的较好的方法。然而，虽然大部分养殖企业严格按照免疫流程接种疫苗，但很多情况下免疫效果并不理想，很多传染病仍不能从根本上得以控制。……