于海英　王涛

[摘要] 目的 探讨不同剂量螺内酯对高血压模型大鼠的心肌保护作用。 方法 选择40只雄性SD大鼠，随机分为对照组、模型组、小剂量组和大剂量组各10只，模型组、小剂量组和大剂量组采用左旋精氨酸甲酯灌胃制作高血压模型，之后仍保持正常饮食，小剂量组给予20 mg/kg螺内酯灌胃，大剂量组给予40 mg/kg螺内酯灌胃，8周后处死，对各组大鼠的心肌病理学图像进行观察分析。 结果 心肌细胞横截面积、小动脉管壁/管腔面积比、心肌内小血管周围纤维化指数均为模型组>小剂量组>大剂量组>对照组，各组间对比差异均具有统计学意义（P<0.05），心肌组织胶原含量模型组、小剂量组和大剂量组均显著高于对照组（P<0.05），模型组显著高于小剂量组和大剂量组（P<0.05），但小剂量组和大剂量组之间对比无显著差异（P>0.05）。 结论 螺内酯能够有效保护高血压模型大鼠的心肌细胞，大剂量应用效果更好，但对心肌胶原含量的影响不大。

[关键词] 高血压；心肌细胞；剂量；螺内酯

[中图分类号] R544.1 [文献标识码] A [文章编号] 1673-9701（2017）24-0030-03

[Abstract] Objective To explore the effect of different doses of spironolactone on the myocardial protection in hypertension model rats. Methods A total of 40 male SD rats were randomly divided into control group， model group， low dose group and high dose group， with 10 rats in each group. In the model group， low dose group and high dose group， gavage of L-arginine methyl ester was used to prepare the hypertension model， and then the rats were maintained the normal diet. The low dose group was given 20 mg/kg of spironolactone gavage， and the high dose group was given 40 mg/kg spironolactone gavage. The rats were executed after 8 weeks. The myocardial pathological images of each group of rats were observed and analyzed. Results The cross-sectional area of myocardial cells， the ratio of small arterial wall/lumen area， and the intramyocardial microvascular fibrosis index all ranked as follows： model group > low dose group > high dose group > control group. The differences between groups were statistically significant（P<0.05）. The content of collagen in cardiac muscle tissue in the model group， low dose group and high dose group was all significantly higher than that in the control group（P<0.05）， and the model group was significantly higher than that in the low dose group and high dose group， but there was no significant difference between the low dose group and the high dose group（P>0.05）. Conclusion Spironolactone can effectively protect the cardiomyocytes of hypertension model rats. The high dose has a better application effect， but has little effect on the content of myocardial collagen.

[Key words] Hypertension； Myocardial cells； Dosage； Spironolactone

高血压是中老年患者最常见的疾病之一，从高血压进展为高血压性心脏病及心力衰竭的过程也是心肌细胞受损和心室重构的过程，在这个过程中肾素-血管紧张素-醛固酮系统被过度激活[1-2]。有研究显示醛固酮作为一种内源性的有害物质，会对心肌细胞造成损害，在心肌细胞纤维化过程中有着重要的作用，同时也是影响心脏功能的重要因素[3]。螺内酯是临床常用的一类醛固酮拮抗剂，具有改善心肌功能的作用[4]。本文就不同剂量螺内酯对高血压模型大鼠的心肌保护作用进行了研究分析，现报道如下。

1 材料与方法

1.1 研究材料

选择40只雄性SD大鼠，均由锦州医科大学实验动物中心提供，体重（114.5±5.9）g，适应性喂养一周后随机分为對照组、模型组、小剂量组和大剂量组各10只。左旋精氨酸甲……