曾庆华+孙小凡+樊琛+齐鑫+李燕+叶勇+刘军海+曾庆梅

摘要：为了优化金樱子（Rosa laevigata Michx.）多糖的提取工艺，采用微波辅助提取方法，以去离子水为溶剂提取金樱子中多糖成分，并以多糖成分提取率为考察指标，通过单因素试验和响应面试验来探讨和优化金樱子多糖的提取工艺，得到金樱子多糖的最佳提取工艺条件为微波时间3.5 min，微波功率438 W，料液比为1∶27（g∶mL），浸提1次。在此提取工艺条件下，金樱子多糖实际提取率可达52.0%。

关键词：金樱子（Rosa laevigata Michx.）；多糖；微波辅助法；单因素试验；响应面试验

中图分类号：TS207.3 文献标识码：A 文章编号：0439-8114（2017）17-3314-06

DOI：10.14088/j.cnki.issn0439-8114.2017.17.032

Optimization of Polysaccharide from Rosa laevigata Michx. Extraction Using Microwave Extraction by Response Surface Methodology

ZENG Qing-hua1，2，SUN Xiao-fan1，FAN Chen1，QI Xin1，LI Yan1，YE Yong2，LIU Jun-hai1，ZENG Qing-mei2

（1.Department of Food Science and Engineering，Liaocheng University，Liaocheng 252059，Shandong，China；

2.School of Food Science and Engineering，Hefei University of Technology，Hefei 230009，China）

Abstract： In order to optimize the extraction process of polysaccharide from Rosa laevigata Michx.，the method of microwave-assisted extraction technology was adopted by using distilled water as the solvent and the extraction rate of polysaccharide as the index. Microwave time，microwave power， ratio of material to liquid and microwave extraction times were studied. Based on the single factor experiment，the extraction process of polysaccharide from Rosa laevigata Michx. polysaccharide was optimized by using the central combination experiment design. The influence factor of the process was determined by regression analysis，and the extraction rate of polysaccharide was determined by the factor of 3. The results showed that the optimum extraction conditions were as follows：the ratio of material to liquid of 1∶27（g∶mL），microwave time of 3.5 min，microwave power of 438 W，extraction of 1 time，the actual extraction rate of polysaccharide was 52.0%.

Key words： Rosa laevigata Michx.；polysaccharide；microwave-assisted extraction；single factor experiments；response surface methodology

金櫻子（Rosa laevigata Michx.），又名糖罐子、白玉带、黄茶瓶、刺头、倒挂金钩等，是蔷薇科蔷薇属的一种野生常绿蔓性灌木。其适宜生长环境对土壤要求不严，阳坡为宜，喜欢温暖湿润和阳光充足的环境，主要分布在中国华东、华中、华南及西南等地[1]，贵州最为丰富。其果实可以入药，具有补肾、利尿的作用，2015年版第一部《中国药典》[2]将金樱子果实收录其中；其叶有消肿、解毒的作用；根药用可以活血散瘀、拔毒驱湿。《本草纲目》记载金樱子“性酸、涩、平，无毒；主治脾泻下痢、止小便利、涩精气；久服，令人耐寒轻身，补血益精有奇效”[3]。在医学上，金樱子可以用于治疗神经衰弱、抑菌消毒、腹泻、高血压病、慢性肾炎、抗动脉粥样硬化、皮肤癌、早期宫颈癌等病症[4-7]。研究表明，金樱子的活性成分可以促进心肌细胞生成，对治疗心肌梗死有良好效果[8]，其提取物对痤疮丙酸杆菌和脂多糖诱导的急性肝损伤也有显著作用[9]。……