张敏敏，赵恒强，赵志国，刘伟，刘倩，梁琰，耿岩玲，黄璐琦，王晓*

(1. 山东省中药质量控制技术重点实验室，山东省科学院中药过程控制研究中心，山东省分析测试中心，山东 济南 250014；2. 中国中医科学院中药资源中心，道地药材国家重点实验室培育基地，中国中医科学院，北京 100700)

【中药与天然活性产物】

微波消解-ICP-MS法测定青海和西藏产区冬虫夏草中的35种元素

张敏敏1，赵恒强1，赵志国1，刘伟1，刘倩1，梁琰1，耿岩玲1，黄璐琦2，王晓1*

(1. 山东省中药质量控制技术重点实验室，山东省科学院中药过程控制研究中心，山东省分析测试中心，山东 济南 250014；2. 中国中医科学院中药资源中心，道地药材国家重点实验室培育基地，中国中医科学院，北京 100700)

为了测定青海和西藏产区的17批冬虫夏草中的35种元素(其中16种为稀土元素)的含量，并进行初步产地鉴别分析，运用微波消解-ICP-MS法测定了3批玉树、4批那曲和10批果洛产区的虫草样本，并对3个产区的测定结果进行聚类分析。结果表明，不同产区的虫草药材各种元素含量差别较大，本文对其中4种有害元素Cd, Pb, As, Cu推荐了限量标准；聚类分析结果表明，玉树和果洛产区聚类较近、差异性较小；那曲产区聚类较远，相对差异性较大。该方法快捷、准确、可行性强，为不同产地冬虫夏草药材的质控和用药安全指导提供了理论依据。

ICP-MS; 冬虫夏草；微量元素；稀土元素

Abstract∶To determine the contents of 35 elements (including 16 rare earth elements) in 17 batches of Chinese caterpillar fungus from Qinghai and Tibet and make preliminary identification analysis of their habitats, a total of 35 elements in Chinese caterpillar fungus samples were determined by microwave digestion-ICP-MS, including 3 batches from Yushu, 4 batches from Naqu and 10 batches from Guoluo. Furthermore, cluster analysis was carried out on the measurement results of 3 producing areas. Results showed that the contents of 35 elements in 17 kinds of Chinese caterpillar fungus samples from different origins have obvious differences. And the limits of the 4 kinds of harmful elements (Cd, Pb, As and Cu) were recommended. The results of cluster analysis showed that the samples from Yushu and Guoluo were close, and the difference was small, while the sample from Naqu was far away, and its relative difference was larger. This method is quick, accurate and feasible, which provides a theoretical basis for quality control and medication guidance of Chinese caterpillar fungus from different habitats.

Key words∶ICP-MS;Chinese caterpillar fungus; trace elements; rare earth elements

冬虫夏草又称虫草，为麦角菌科真菌冬虫夏草菌Cordycepssinensis(Berk. ) Sacc. 寄生在蝙蝠蛾科昆虫幼虫上的子座和幼虫尸体的干燥复合体[1]。……