杨秀青，谷江华，石征蓉，袁强华，宋英#，何成诗

（1.成都中医药大学药学院，成都610075；2.成都中医药大学附属医院药剂科，成都 610072）

银菊解毒口服液的水提工艺研究

杨秀青1＊，谷江华1，石征蓉1，袁强华2，宋英2#，何成诗2

（1.成都中医药大学药学院，成都610075；2.成都中医药大学附属医院药剂科，成都 610072）

目的：对银菊解毒口服液水提工艺进行优化，为该制剂的工业化生产提供参考。方法：通过对银菊解毒方中的绿原酸提取时间-提取率曲线及川银花与方中其他药味配伍合煎后绿原酸的提取率进行考察，确定川银花的煎煮方式及时间；以蒙花苷、哈巴俄苷、（R，S）-告依春、补骨脂素+异补骨脂素含量及干膏率的综合评分为指标，以加水量、煎煮时间、煎煮次数为考察因素设计L9（34）正交试验，优化川银花药渣与其余药味的提取工艺并进行验证试验。结果：最优水提工艺为川银花加8倍量水先煎30 min，留存药液；药渣与其余药味加8倍量水煎煮3次，每次1 h；所有药液合并。验证试验中绿原酸、蒙花苷、哈巴俄苷、（R，S）-告依春、补骨脂素+异补骨脂素含量平均值依次为34.51、10.31、1.97、0.21、9.79 mg/g（RSD分别为1.24%、1.19%、1.40%、1.71%、1.28%，n＝3），干膏率平均值为25.4%（RSD＝1.64%，n＝3），绿原酸平均提取率为78.95%（RSD＝1.24%，n＝3）。结论：优化的水提工艺中绿原酸的提取率及其他药效成分的含量均较高，且工艺稳定、可行。

银菊解毒口服液；绿原酸；提取时间；提取率；配伍合煎；正交试验；水提工艺

ABSTRACTOBJECTIVE：To optimize the water extraction technology of Yinju jiedu oral liquid，and provide reference for the industrial production of the preparation.METHODS：According to the investigation of extraction time-extraction rate curves of chlorohenic acid of Yinju jiedu formula and extraction rate of chlorohenic acid in Lonicera japonica and other combined medicinal materials in the formula，decoction methods and time of L.japonica were determined.Using the comprehensive scores of linarin，harpagoside，（R，S）-epigoitrin，psoralen+angelicin contents and dry extraction yield as indexes，L9（34）orthogonal test was designed to detect the effects of adding water amount，decoction time times and optimize the extraction technology of the residues and other medicinal materials.Verification test was conducted.RESULTS：The optimal technology was L.japonica decocted first for 30 min with 8-fold water；the residues and other medicinal materials were decocted with 8-fold water for 3 times，1 h each time；combining all the syrups.In verification test，the average contents of chlorohenic acid，linarin，harpagoside，（R，S）-epigoitrin，psoralen+angelicin were respectively 34.51，10.31，1.97，0.21，9.79 mg/g（RSD＝1.24%，1.19%，1.40%，1.71%，1.28%，n＝3）；average dry extraction yield was 25.4%（RSD＝1.64%，n＝3）；average extraction rate of chlorohenic acid was 78.95%（RSD＝1.24%，n＝3）.CONCLUSIONS：In the optimized water extraction technology，both the extraction rate of chlorohenic acid and contents of other ingredients are relatively high.The technology is stable and feasible.

KEYWORDSYinju jiedu oral liquid；Chlorogenic acid；Extraction time；Extraction rate；Compatibility decoction；Orthogonal test；Water extraction technology

银菊解毒口服液为在研中药新制剂，源自成都中医药大学附属医院治疗牙龈炎、牙周炎应用了二十余年的临床经验方。全方由川银花、野菊花等5种药味组成。……