刘颖 唐家年 黄川腾 刘国选 林诗婉 高美芳 吴东霖 陈建平

摘要 [目的]建立促进麻疯树再生不定芽生根的组培体系。[方法] 通过在诱导麻疯树再生不定芽分化生根的培养基（MS+0.3 mg/L IBA）中添加一定浓度的L-谷氨酰胺（Gln）来达到促进芽条生根的效果。[结果] 在诱导麻疯树再生不定芽分化生根的培养基中添加20 mg/L Gln，可以显著提高芽条的生根率，生根率可达40%～50%。同时，可以在短时间内就获得较高的生根率，显著縮短了获得完整植株的周期。此外，还减少了生根过程中常见的再生植株叶片发黄、容易脱落现象的发生，显著提高了所获得的再生完整植株的质量。[结论] 麻疯树生根培养基的最优组合为添加0.3 mg/L IBA和20 mg/L Gln的MS培养基，应用该研究建立的组培体系可显著改善麻疯树再生不定芽的生根效果。

关键词 麻疯树；L-谷氨酰胺；再生不定芽；生根

中图分类号 S184；Q949.9 文献标识码 A 文章编号 0517-6611（2017）07-0125-05

Effect of L-Glutamine on the Rooting of Regenerated Shoot Buds of Jatropha curcas

LIU Ying1，TANG Jia-nian1，HUANG Chuan-teng2，CHEN Jian-ping3* et al （1.Faculty of Agricultural Science，Guangdong Ocean University，Zhanjiang，Guangdong 524088；2.Hainan Provincial Forestry Institute，Haikou，Hainan 571100；3.Faculty of Food Science and Technology，Guangdong Ocean University，Zhanjiang，Guangdong 524088）

Abstract [Objective] To establish a tissue culture system for promoting rooting of regenerated buds of Jatropha curcas.[Method] L-glutamine （Gln） was added into rooting medium （MS+0.3 mg/L IBA） for improving the rooting efficiency of regenerated buds of J.curcas.[Result]When 20 mg/L Gln was supplemented into rooting medium，the rooting efficiency of regenerated shoot buds could be significantly increased （raised up 40%-50%）.In addition，satisfactory rooting rate was obtained in a short time （10～20 days），and the cycle of gaining a complete plant was shortened significantly.Moreover，reducing the chances of this happening that the leaves were easy to be yellowing and fall down from regenerated plants in the process of rooting，and then the quality of regenerated whole plants was obviously improved.[Conclusion] The optimal combination of rooting medium was MS medium supplemented with 0.3 mg/L IBA and 20 mg/L Gln.The rooting effect of regenerated buds of J.curcas could be significantly enhanced via application of the tissue culture system established in this study.

Key words Jatropha curcas；L-glutamine；Regenerated shoot buds；Rooting

麻疯树（Jatropha curcas L.）又名小桐子、黄肿树，为大戟科（Euphorhiaceae）麻疯树属（Jatropha）多年生的落叶灌木或小乔木[1]。麻疯树原产于中美洲，主要分布于热带和亚热带地区[2]，在我国主要生长在四川、云南、贵州、广东、广西、海南和台湾等省（区）。麻疯树组织提取物中的毒蛋白、麻疯酮等活性成分可以被开发为药物，常用于消肿散淤、清热解毒等，近年来还发现这些物质具有显著的抗癌活性[3-4]，还可用于生物病虫害防治[5]。麻疯树种子含油量高达40%～60%，提取的种子油可用于生产优质的“生物柴油”[6-8]，有利于缓解当前全球石油能源短缺的问题。麻疯树被认为是一种重要的可再生能源，受到世界各国科学家的广泛关注[7-8]。……