靳欣欣 田英姿

摘要 [目的]優化大叶白麻茎多酚的提取工艺，并考察其体外抗氧化性。[方法]采用响应面法对大叶白麻茎多酚的提取工艺进行了优化，考察了料液比、超声强度、超声时间对提取得率的影响，以VC为参照，将大叶白麻茎多酚和茶多酚的抗氧化性进行对比。[结果]大叶白麻茎多酚的最佳提取工艺是料液比1∶17，超声强度410 W，提取时间60 min，在该条件下大叶白麻茎多酚的提取得率为2.09%，提取时间和超聲强度对提取得率的影响均为极显著。还原力与还原剂浓度之间有较好的量效关系，三者的还原力从高到低依次为大叶白麻茎多酚、茶多酚、VC，与抗氧化能力的强弱性一致。大叶白麻茎多酚清除羟基自由基的能力是茶多酚的1.34倍，清除超氧阴离子、DPPH·自由基的能力与茶多酚较为接近，且明显高于VC，分别是VC的2.00、2.80倍。[结论]大叶白麻茎多酚具有良好的抗氧化活性，应加强对其进一步的应用研究。

关键词 大叶白麻茎；多酚；抗氧化；响应面

中图分类号 TS01 文献标识码 A 文章编号 0517-6611（2017）07-0116-04

Study on Optimization of Extraction Process of Polyphenol in Stem of Poacynum hendersonii by Response Surface Aanlysis and Its Oxidation Resistance

JIN Xin-xin1，TIAN Ying-zi1，2*

（1.College of Light Industry and Food Sciences，South China University of Technology，Guangzhou，Guangdong 510640； 2.College of Life and Geography Sciences，Kashi University，Kashi，Xinjiang 844006）

Abstract [Objectuve] The aim of the study was to optimize extraction technology of polyphenol in stem of Poacynum hendersonii and study its antioxidation in vitro.[Method]The extraction technology of polyphenol was optimized by response surface analysis and the effect of liduid ratio，extraction time and ultrasonic intensity on extraction yield of polyphenol was investigated.VC taken as reference，antioxidant activity of polyphenol in Poacynum hendersonii and tea were compared and analyzed.[Result] The optimal extraction technology was as follows solid-liquid ratio of 1∶17，ultrasonic intensity of 410 W，extraction time for 60 min，under this technology，the extraction yield of polyphenol from Poacynum hendersonii was 2.09%.Both influence of time and ultrasonic intensity on the yield was very significant.The order of antioxidative effect from high to low was polyphenol in Poacynum Hendersonii，polyphenol in tea，VC.As the dose of the three antioxidant increasing，reducing power strengthens，displays good does-effect relationship.The order of reducing power and antioxidative effect were identical.Hydroxyl radical scavenging ability of polyphenol in Poacynum Hendersonii was about 1.34 times of polyphenol in tea.DPPH· and superoxide anion scavenging ability of polyphenol in stem of Poacynum hendersonii were close to that in tea，2.00，2.80 times of VC.[Conclusion]The polyphenol in stem of Poacynum hendersonii has been shown to exhibit excellent antioxidant activity，so it should strengthen the further application research.

Key words Poacynum Hendersonii；Polyphenol；Antioxidant activity；Response surface

大叶白麻（Poacynum hendersonii），又称大花罗布麻，直立半灌木，主要分布于新疆、青海和甘肃等省区。主要野生在盐碱荒地和沙漠边缘及河流两岸冲积平原水田和湖泊周围[1]。大叶白麻叶被用于冲泡和中药，但其茎部被大量丢弃。目前有大叶白麻多酚提取和抗氧化性研究的相关报道[2-3]，而关于大叶白麻茎中多酚的提取工艺及抗氧化性鲜见报道。笔者运用响应面法优化提取大叶白麻茎多酚，以VC为参照，将大叶白麻茎多酚和茶多酚的抗氧化性进行对比，旨在为大叶白麻茎多酚的推广应用提供科学依据。……