张相伦++范喜梅++游伟++成海建++靳青

摘要：本试验旨在研究饲粮添加不同水平β-胡萝卜素对肉牛血液抗氧化性能的影响。选用120头西门塔尔牛与鲁西黄牛杂交牛阉牛，平均体重为（381.00±26.01）kg，随机分为4组。经过15 d预试期后，各组添加不同水平β-胡萝卜素（分别为每头牛0、600、1 200、1 800 mg/d），添加90 d后进行为期60 d（每30 d为一个阶段）的跟踪测试，各组均饲喂基础日粮。各试验阶段结束时（90、120、150 d），对试验牛进行采血并对血清中β-胡萝卜素和维生素A含量以及抗氧化指标进行测定。结果表明，日粮添加β-胡萝卜素可显著提高血清中β-胡萝卜素含量，停止添加后血清β-胡萝卜素含量逐渐降至对照组水平；日粮添加β-胡萝卜素对血清维生素A含量无显著影响。日粮添加β-胡萝卜素后，血清中总抗氧化能力（T-AOC）、谷胱甘肽（GSH）含量、总超氧化物歧化酶（T-SOD）活性均显著提高，丙二醛（MDA）含量显著降低，停止添加后，各项抗氧化指标逐渐恢复至对照组水平。总之，日粮添加β-胡萝卜素可显著影响肉牛抗氧化功能。

关键词：肉牛；β-胡萝卜素；血清；抗氧化性

中图分类号：S823.9+2文献标识号：A文章编号：1001-4942（2017）06-0119-04

AbstractThis study was conducted to determine the effects of adding different levels of β-carotene （βC） into ration on the serum antioxidant activity of beef cattle. Total 120 Simmental and Luxi crossbred steers with mean live weight of （381.00 ± 26.01） kg were randomly divided into 4 groups. After an adjustment period for 15 days， each group was supplemented with βC at 0， 600， 1 200 or 1 800 mg/d per steer for 90 days， and then the tracking test was conducted with basic ration for 60 days （including two sub-periods， each for 30 days）. At the end of each period （the 90th， 120th and 150th day）， the venous blood of tested cattles were collected， and the βC and vitamin A （V〖WT6〗A〖WT5〗） contents and antioxidant indices in serum were analyzed. The results showed that βC added into ration significantly increased the serum βC content， and then reduced to the control level after stopping supplemention. No significant difference in serum V〖WT6〗A〖WT5〗 was detected during the experimental period. The serum total antioxidant capacity （T-AOC）， glutathione （GSH） content and total superoxide dismutase （T-SOD） activity all increased， whereas the serum malondialdehyde （MDA） content decreased after βC supplementation. During the tracking test period， all values of antioxidant indices renewed to the control level gradually. In conclusion， βC added into ration affected antioxidant function of beef cattle.

KeywordsBeef cattle； β-carotene； Serum； Antioxidant activity

類胡萝卜素是自然界中一类重要的天然色素，为动物体内维生素A（VA）的主要来源，动物自身没有合成VA的功能，主要通过裂解具有VA原活性的类胡萝卜素获得。在所有的VA原类胡萝卜素中，β-胡萝卜素（βC）分解为VA的效率最高，而且牛主要吸收β-胡萝卜素[1]。动物机体需要通过维持体内自由基的生理平衡来维持多项生理功能的正常进行，这种平衡一旦被打破，各种生理功能都会受影响。……