张建华 查玉平 陈京元 王义勋 赵东容 唐桂林

摘要：在白僵菌三级生产中，使用3种不同培养盘，研究其对原菌粉生产的影响。结果表明，多孔塑盘生产的原菌粉含孢量和活孢率最高，分别为12225±217亿孢子/g和9050±096%。多孔塑盘生产的原菌粉显著好于竹制簸箕和镀锌钢盘生产的原菌粉。而且多孔塑盘在通透性和操作性上具有优势，烘干所需时间最短。这说明多孔塑盘适合用于白僵菌的三级生产。

关键词：白僵菌；培养盘；工业化生产；含孢量；活孢率

中图分类号：文献标识码：A文章编号：1004-3020（2016）06-0040-03

Effect of Deferent Culture Plates on Production of Beauveria bassiana

Zhang Jianhua（1）Zha Yuping（2）Chen Jingyuan（2） Wang Yixun（2）Zhao Dongrong（1）Tang Guilin（1）

（1.Dangyang City Station for Forest Pest and Disease Control and QuarantineYichang443000；2. Hubei Academy of ForestryWuhan430075）

Abstract： In tertiary production of Beauveria bassiana， effects of three different culture plates on the raw powder production were studied. The results showed that the spore density and viabilty of the raw powder， which produced in porous plastic plates， were highest. The spore density was 122.25±2.17 billion spores/g， and viability was 90.50±0.96%. The raw powder produced in porous plastic plates significantly better than those produced in bamboo dustpan or galvanized steel plate. And porous plastic plates had advantages in permeability and workability， in which the raw powder drying needed fewest time. These showed that porous plastic plates were suitable to tertiary production of B. bassiana.

Key words：Beauveria bassiana；Culture plate；industrial production；spore density；viabilty

白僵菌屬于丝孢纲Hyphomycetale 丛梗孢目Moniliales 丛梗孢科Moniliaceae 白僵菌属Beauveria，是一类寄主范围广、致病力强的昆虫病原真菌[1]。我国已经将白僵菌广泛应用于农林害虫的防治当中，并取得了较好效果，尤其在玉米螟Ostrina furnacalis和马尾松毛虫Dendrolimus punctatus防治上，取得了令人瞩目的经济效益和生态效益[2-4]。在目前农林害虫防治中，白僵菌是应用最多的一种真菌杀虫剂。

早在上世纪70年代，我国就开始大量生产白僵菌。当时主要为固体发酵，即从一级到三级均用固体培养基进行培养[5]。到上世纪80年代，我国白僵菌生产有了较大进展， 分生孢子提取技术改进为旋风分离机等机械提取[6]。近年来，我国白僵菌生产工艺主要为液固两相发酵，利用液体发酵快速生产大量的菌丝体或芽生孢子，再转到固体营养基质上使其产生气生分生孢子[7]。其中，三级生产是白僵菌生产工艺中变革较多的一个环节，例如由土床发酵发展到浅盘发酵[7]。……