汪洁 朱顺娟 樊秦 杨丽 夏鹏飞 王引权

摘要：目的 建立甘肃不同产地黄管秦艽药材的灰色关联度模型，并进行质量评价。方法 以甘肃10个产地黄管秦艽为研究对象，测定龙胆苦苷、总环烯醚萜苷类、醇浸出物、总灰分、酸不溶性灰分含量，采用灰色关联度法构建黄管秦艽质量的灰色关联度评价模型。結果 10个不同产地黄管秦艽样品的相对关联度在0.394～0.652之间。其中相对关联度>0.50的样品有6个产地，其样品质量评价较高，其余产地药材质量评价较低（相对关联度<0.50）。卓尼县柳林乡观景台产地黄管秦艽的指标相对关联度最大（0.652），质量评价最优；漳县三岔镇狼王沟产地黄管秦艽的指标相对关联度最小（0.394），质量评价最差。结论 灰色关联度分析法及模型可应用于黄管秦艽药材的质量评价，本研究结果可为黄管秦艽的开发利用提供一定的试验基础。

关键词：黄管秦艽；灰色关联度法；质量评价

DOI：10.3969/j.issn.1005-5304.2017.03.017

中图分类号：R284.1 文献标识码：A 文章编号：1005-5304（2017）03-0069-06

Quality Evaluation of Gentiana officinalis H.Smith. from Different Producing Areas of Gansu Province by Grey Incidence Degree Method WANG Jie1， ZHU Shun-juan1， FAN Qin2， YANG Li1， XIA Peng-fei3， WANG Yin-quan4， ZHAO Lei1，2，5 （1. Gansu University of Chinese Medicine， Lanzhou 730000， China； 2. Key Laboratory for Traditional Chinese （Tibetan） Medicine Chemistry and Quality Control of Gansu University， Lanzhou 730000， China； 3. Key Laboratory of Pharmacology and Toxicology of TCM of Gansu Province， Lanzhou 730000， China； 4. Northwest Collaborative Innovation Center for Chinese Medicine， Lanzhou 730000， China； 5. Cultivation Base for Key Laboratory of Chinese Medicine Quality and Standard of Gansu Province， Lanzhou 730000， China）

Abstract： Objective To establish the degree of gray incidence model of Gentiana officinalis H.Smith. from differenct producing areas of Gansu Province and conduct quality evaluation. Methods Ten samples of Gentiana officinalis H.Smith. from different producing areas in Gansu Province were collected and the gentiopicroside， total iridoid gycoside， extractives， total ash， acid-insoluble ash content of Gentiana officinalis H.Smith were tested. The quality evaluation of grey incidence model of Gentiana officinalis H.Smith. was established by grey incidence model. Results The relative correlation degrees of all evaluation items were in the range of 0.394–0.652. Among them， the relative correlation degree of samples from 6 producint areas were greater than 0.50， which had high quality evaluation. The relative correlation degrees of the rest samples from other producing areas were less than 0.50， which had low quality evaluation. The sample of Gentiana officinalis H.Smith. from Zhuoni County Liulin Township Observatory had the most highly correlated with the optimal reference sequence （0.652） and so the best in quality； The sample of Gentiana officinalis H.Smith. from Zhang County Sancha Town Langwang Ditch had the most lowly correlated with the optimal reference sequence （0.394） and so the worst in quality. Conclusion This method and quality evaluation model can be used for the quality evaluation for Gentiana officinalis H.Smith.， which can provide a

基金项目：国家自然科学基金（81660577）；甘肃省属高校基本科研业务费专项基金（2011-06）；甘肃省中药药理与毒理学重点实验室自主基金项目（ZDSYS-ZZ-2015-001）；西北中藏药协同创新中心重点项目（2015-05）；甘肃中医学院中青年科研基金项目（ZQ-2014-2）

通讯作者：赵磊，E-mail：zzyhx@gszy.edu.cn

certain experimental basis for the development and utilization of new resources.

Key words： Gentiana officinalis H.Smith.； grey incidence degree method； quality evaluation

2015年版《中华人民共和国药典》规定，秦艽为龙胆科龙胆属多年生草本植物秦艽Gentiana macrophylla Pall.、麻花秦艽Gentiana straminea Maxim.、粗茎秦艽Gentiana crassicaulis Duthie ex Burk.和小秦艽Gentiana dahurica Fisch.的干燥根[1]。秦艽始载于《神农本草经》：“主寒热邪气，寒湿风痹，肢节痛、下水、利小便。”其味辛、苦，性平，归胃、肝、胆经，可祛风湿、舒筋络、退虚热、清肝胆湿热而退黄，用于风湿痹痛、骨蒸潮热、骨节酸痛、筋脉拘挛、湿热黄疸等症[2]。甘肃为秦艽的道地产区，早在1987年秦艽被国家列为濒危三级保护物种[3]。非药典品种黄管秦艽其药材性状、根部横切面、显微特征结构均与秦艽相似，黄管秦艽与秦艽在ITS水平上为一类，同源性达99.5%[4]。黄……