杜学铅++周勇++齐攀

[摘要] 目的 研究MTH1與乳腺肿瘤的相关性。方法 选取2014年5月～2015年7月新乡市中心医院57例乳腺蜡块及部分组织。按乳腺良性肿瘤和乳腺恶性肿瘤分成两组，分别为20例和37例。通过免疫组化的方法检测两组组织实质、间质中MTH1的含量。将恶性肿瘤组织按激素受体（HR）（HR包括雌激素和孕激素受体）阴性、阳性；淋巴结有无转移；Ki-67>20%、≤20%；人表皮生长因子受体2（HER-2）（-）、HER-2（+～+++）分别分两组，通过免疫组化方法检测各组实质、间质中MTH1的含量。进一步应用Western blot方法检测乳腺肿瘤组织中MTH1的表达差异。结果 MTH1在乳腺肿瘤实质及间质中的含量，恶性肿瘤明显高于良性肿瘤，差异有统计学意义（P < 0.05）；MTH1在HR阳性和HR阴性的乳腺恶性肿瘤组织中的含量差异无统计学意义（P > 0.05）；MTH1在有淋巴结转移和无淋巴结转移的乳腺恶性肿瘤组织中含量差异无统计学意义（P > 0.05）；MTH1在乳腺恶性肿瘤实质及间质中的含量，Ki-67>20%组中明显高于Ki-67≤20%组，HER-2（+～+++）组中明显高于HER-2（-）组，差异有统计学意义（P < 0.05）。结论 乳腺恶性肿瘤组织中MTH1明显高于乳腺良性组织，并且恶性程度与MTH1含量呈正相关。

[关键词] 乳腺恶性肿瘤细胞；乳腺良性肿瘤细胞；MTH1；免疫组化

[中图分类号] R737.9 [文献标识码] A [文章编号] 1673-7210（2017）01（b）-0102-04

Study on the correlation between MTH1 and mammary tumor

DU Xueqian1 ZHOU Yong2 QI Pan2 YUAN Longjian1 HENG Ruijuan1 YANG Liuqin2

1.Xinxiang Medical University， He'nan Province， Xinxiang 453000， China； 2.Department of Oncological Surgery， Xinxiang Central Hospital， He'nan Province， Xinxiang 453000， China

[Abstract] Objective To study the correlation between MTH1 and mammary tumor. Methods Fifty-seven paraffin-embedded samples of breast and some breast tissue obtained from Xinxiang Central Hospital from May 2014 to July 2015 were selected. They were divided into two groups， including mammary benign tumor and mammary malignant tumor， which contained 20 cases and 37 cases respectively. The content of MTH1 in breast parenchyma and mesenchyme was assayed by immunohistochemical staining. The malignant tumor group was divided into two groups respectively based on positive or negative hormone receptor （HR） （HR included estrogen receptor and progestrone receptor）， lymphatic metastasis or not， Ki-67>20% or ≤20%， human epidermal growth factor recptor 2 （HER-2） （-） or HER-2 （+-+++）， the content of MTH1 in breast parenchyma and mesenchyme was assayed by immunohistochemical staining. Western blot was used to detect the expression of MTH1 in breast tumor tissues. Results For the content of MTH1 breast parenchyma and mesenchyme， the mammary malignant tumor was significantly higher than that in mammary benign tumor， the difference was statistically significant （P < 0.05）. There was no significant difference of the content of MTH1 in breast malignant tumor between positive HR and negative HR （P > 0.05）. For the content of MTH1 in parenchyma and mesenchyme of breast malignant tumor， Ki-67>20% group was higher than that of Ki-67≤20% group， HER-2 （+-+++） group was higher than that of HER-2 （-） group， the differences were statistically significant （P < 0.05）. Conclusion The content of MTH1 in mammary malignant tumor is significantly higher than that in mammary benign tumor， and the content of MHT1 is positively correlated with the malignant grade of mammary tumor.

[Key words] Mammary malignant tumor； Mammary benign tumor； MTH1； Immunohistochemical staining

乳腺癌是女性最常见的恶性肿瘤之一，随着经济的不断发展，生活水平的不断提高，乳腺癌（breast cancer）发病率逐年增长，发病率一直处于上升趋势，2012年，全球约有170万例乳腺癌新发病例和52.19万例乳腺癌死亡病例[1]。中国乳腺癌发病率以每年3%的水平持续增长，一些大城市的乳腺癌发病率已经居于女性恶性肿瘤首位[2]。肿瘤细胞在不断复制过程中会产生大量活性氧族（ROS），而ROS可导致NTP氧化，DNA复制过程中用到NTP，如果氧化的NTP渗入到DNA中，合成出来的核酸会导致基因组不稳定[3-4]，会导致基因突变，这是所有癌症分子的基础部分，这是一种破坏性的损伤，导致突变及细胞死亡[5-7]。研究表……