李慧玲++吴骁++王丹

[摘要] 目的 观察木犀草苷对3T3-L1前脂肪细胞分化的抑制作用。 方法 用噻唑蓝（MTT）法检测木犀草苷对3T3-L1前脂肪细胞增殖的影响；用鸡尾酒法诱导3T3-L1细胞分化，用油红O染色、三酰甘油含量检测脂肪细胞分化程度；采用RT-PCR和Western blot方法检测木犀草苷对脂肪细胞过氧化物酶体增殖物活化受体γ（PPARγ）、CCAAT/增强子结合蛋白α（C/EBPα）mRNA和蛋白表达的影响。 结果 木犀草苷5～80 μmol/L不影响细胞活性，160～320 μmol/L显著抑制细胞活性，呈剂量依赖性抑制3T3-L1前脂肪细胞的分化，显著降低脂肪细胞PPARγ、C/EBPα mRNA和蛋白的表达。 结论 木犀草苷能抑制3T3-L1前脂肪细胞分化，减少脂质聚集，是通过下调PPARγ、C/EBPα的表达实现的。

[关键词] 木犀草苷；3T3-L1前脂肪细胞；分化；PPARγ；C/EBPα

[中图分类号] R285 [文献标识码] A [文章编号] 1673-7210（2017）01（b）-0016-04

Inhibitory effect of galuteolin on the differentiation of 3T3-L1 preadipocyte

LI Huiling1 WU Xiao2 WANG Dan3 ZHANG Qiuhua1

1.Staff Room of Basic Pharmacology， Liaoning University of Traditional Chinese Medicine， Liaoning Province， Shenyang 110847， China； 2.Experimental Animal Center， He University， Liaoning Province， Shenyang 110163， China； 3.Staff Room of Histology and Embryology， Basic Medical College， Jinzhou Medical University， Liaoning Province， Jinzhou 121001， China

[Abstract] Objective To observe the inhibitory effect of galuteolin on the differentiation of 3T3-L1 preadipocyte. Methods The effect of galuteolin on the proliferation of 3T3-L1 preadipocyte was detected by methyl thiazolyl tetrazolium （MTT）； the differentiation of 3T3-L1 preadipocyte was induced by the cocktail method， the differentiation degree of preadipocyte was detected by oil red O staining and the content of triacylglycerol； the effects of galuteolin for the mRNA and protein expression of peroxisome proliferator activated receptor-γ （PPARγ） and CCAAT/enhancer binding protein α （C/EBPα） were measured by RT-PCR and Western blot. Results The concentration of 5-80 μmol/L galuteolin did not affect the cell activity， 160-320 μmol/L galuteolin could significantly inhibit the cell activity， which could significantly inhibit the differentiation of 3T3-L1 preadipocyte in a dose-dependent manner and decrease the mRNA and protein expression of PPARγ and C/EBPα. Conclusion Galuteolin can inhibit the differentiation of 3T3-L1 preadipocyte， reduce the lipid accumulation， which may be realized by down-regulating the expression of PPARγ and C/EBPα.

[Key words] Galuteolin； 3T3-L1 preadipocyte； Differentiation； PPARγ； C/EBPα

肥胖是高血壓病、2型糖尿病、心血管疾病、癌症等的高危诱发因素，已成为快速增长并蔓延全球的公共健康威胁[1-5]，它是由遗传、环境等特定因素引起的进食调控和能量代谢紊乱，使脂肪积聚过多、体重超常的内分泌代谢疾病。近年来研究发现，姜黄素、白藜芦醇、槲皮素等黄酮类化合物有较好的减肥作用[7]。木犀草苷是豆茶决明、金银花、荆芥等植物中的天然黄酮类化合物[6]。笔者前期研究发现，豆茶决明有减肥作用[8]，其中包含黄酮类化合物木犀草苷。本研究主要探讨木犀草苷对3T3-L1前脂肪细胞分化的影响及机制。

1 材料与方法

1.1 主要仪器

VARIOSKAN FIASH酶标仪（Thermo Scientific）；CKX41倒置生物显微镜（中国OLYMPUS有限公司）；HERAcell 150i细胞培养箱（Thermo Scientific）；YJ-VS超净工作台（天锡一净净化设备有限公司）；Hema480基因扩增仪（珠海黑马医学仪器有限公司）；……