马玲 问荣荣 邱本军 王步勇 刘雪峰

(东北林业大学，哈尔滨，150040)



黄芪多糖提高小叶杨抗病性1)

马玲 问荣荣 邱本军 王步勇 刘雪峰

(东北林业大学，哈尔滨，150040)

研究了不同质量浓度黄芪多糖处理过的小叶杨(Populussimonii)在接种杨树烂皮病菌(Valsasordida)后其体内与抗性有关的物质含量和酶活性的变化，探究了黄芪多糖增强植物抗病性的作用机制。结果显示：黄芪多糖对杨树烂皮病菌菌丝生长具有抑制作用；低质量浓度黄芪多糖在短时间内能够提高小叶杨叶片内可溶性糖质量分数，而高质量浓度黄芪多糖可使小叶杨叶片内可溶性糖质量分数缓慢持续增加；0.010 g·L-1黄芪多糖处理后，叶片内可溶性蛋白质量分数和苯丙氨酸解氨酶活性均显著高于对照组；而总酚活性在0.100 g·L-1黄芪多糖处理组中达最高；黄芪多糖各质量浓度处理后，叶片内叶绿素a、b及总量均显著高于对照组。黄芪多糖能够通过提高与抗性相关的物质的含量及酶的活性来提高林木自身抗性，从而促进林木的生长发育。

黄芪多糖；小叶杨；杨树烂皮病菌；抗病性

To explore the mechanism ofPopulussimoniitreated with Astragalus polysaccharide in enhancing the disease resistance, we measured the concentration of active substances and the activities of enzymes in simonii seedlings sprayed with Astragalus polysaccharides and then inoculated withValsasordida. Astragalus polysaccharide could inhibited the growth ofV.sordida. Low concentrations of Astragalus polysaccharides could improve the soluble sugar content in leaf of simonii seedlings in a short period of time, while the soluble sugar was increased slowly and continually for a long time at high concentrations of Astragalus polysaccharides. Astragalus polysaccharides of 0.01 mg/mL could significantly improve soluble protein content and PAL activity inP.simonii, compared with the control. After treated with 0.1 mg/mL Astragalus polysaccharide, the total phenolic content inPopulussimoniireached the highest. Different concentrations of Astragalus polysaccharid improved the content of chlorophyll inP.simonii, and were significantly higher than the control group. Astragalus polysaccharide could raise the substances content and enzyme activity inP.simoniirelated to the resistance to improve the resistance of trees, and thus promote the growth of trees.

杨树(Populusspp.)是世界上分布最广泛的树种，其天然种有100多种[1]。我国杨树资源丰富，天然种约50种，且分布面积广。由于轮伐期短、根系发达、树冠繁茂，能够减少水土流失，使杨树成为速生丰产林的主要树种，并在生态、工业和生活上具有多种用途[2]。近年来，随着林业科技成果的广泛应用，杨树新品种的大力推广，杨树栽植面积的扩大及杨树人工林营造面积的迅速增加，使得病害发生日趋严重，给林业生产和生态建设带来巨大的损失。……