刘庭庭， 马　洪， 段晓峰

(贵州医科大学 口腔医学系， 贵州 贵阳　550004)



Bmi-1在口腔鳞癌组织中的表达及意义*

刘庭庭， 马洪**， 段晓峰

(贵州医科大学 口腔医学系， 贵州 贵阳550004)

目的： 探讨Bmi-1基因在口腔鳞癌组织中的表达及意义。方法： 收集口腔鳞癌(OSCC)患者手术切除新鲜标本30例作为OSCC组，癌旁0.5 cm外正常组织及正常体检者的口腔黏膜组织共30例作为正常对照组，免疫组织化学染色SP法检测两组组织中Bmi-1蛋白的表达，实时定量基因扩增荧光检测系统(QPCR)检测Bmi-1 mRNA的表达，比较不同临床理特征OSCC患者Bmi-1的表达差异。结果： OSCC组织中，Bmi-1蛋白和mRNA的表达明显高于正常对照组；在OSCC组织中，中低分化的OSCC组织的Bmi-1蛋白和mRNA表达高于高分化OSCC组织，差异具有统计学意义(P<0.05)，Bmi-1表达与患者年龄、性别无关(P>0.05)。结论： Bmi-1基因检测可作为OSCC的辅助诊断指标之一。

Bmi-1； 基因； 口腔肿瘤； 癌,鳞状细胞； 免疫组织化学

[Abstract]Objective: To explore the Bmi-1 gene expression and clinical significance in oral squamous carcinoma(OSCC) tissues. Methods: Fresh tissue samples of 30 patients with oral squamous cell carcinomas were collected as OSCC group, while 30 cases of 0.5 cm normal tissue adjacent to carcinoma and normal physical examination were taken as the normal control group. The expression of Bmi-1 protein in the two groups was detected by immunohistochemistry SP method, and the expression of mRNA Bmi-1 was detected by real-time quantitative PCR. Results: The expression of Bmi-1 protein and mRNA in OSCC tissue were significantly higher than those in normal control group. In OSCC tissues, the expression of Bmi-1 protein and mRNA in the low and middle differentiated OSCC tissues were higher than those in the highly differentiated OSCC tissues, and the difference was statistically significant (P<0.05). BMI-1 expression was not related to patient's age and sex. Conclusion: Bmi-1 gene detection can be used as one of the auxiliary diagnostic indicators of oral squamous cell carcinomas.

[Key words]Bmi-1; gene; mouth neoplasms; carcinoma; squamous cell，immunohistochemistry

PcG蛋白-多梳家族蛋白(polycomb groupprotein)是一类在染色质水平抑制靶基因转录的蛋白，在胚胎发育、细胞记忆、细胞周期、细胞增殖和肿瘤形成等过程中起到非常重要的作用。B细胞特异性莫洛尼鼠白血病病毒整合位点(B-cell-speciifc moloney murine leukemia virus integration site 1，Bmi-1)是目前研究中最深入的哺乳动物PcG家族成员。近年来，越来越多的证据表明 ，Bmi-1在恶性肿瘤的发生发展中具有重要作用。Bmi-1在很多肿瘤中处于高表达的状态，如髓母细胞瘤[1]、非霍奇金淋巴瘤[2]、肝癌[3]、结直肠癌[4]、乳腺癌[5]等,本研究通过检测口腔鳞癌(oral squamous cell carcinoma，OSCC)组织中Bmi-1的表达，并比较OSCC不同临床病理特征患者Bmi-1表达的差异，为OSCC的临床早期诊断、生物蛋白靶向治疗及预后监测判断提供理论基础。……