杨燕平， 高晓勤， 杨　喆， 刘　智， 马晓萍， 丁贤胜, 秦海霞

(1．贵州医科大学 组织学与胚胎学教研室， 贵州 贵阳　550004； 2．遵义医药高等专科学校， 贵州 遵义　563002； 3．六盘水市人民医院 病理科， 贵州 六盘水　553001)



亚慢性砷染毒对大鼠睾丸组织的影响*

杨燕平1， 高晓勤2**， 杨喆1， 刘智1， 马晓萍2， 丁贤胜3, 秦海霞1

(1．贵州医科大学 组织学与胚胎学教研室， 贵州 贵阳550004； 2．遵义医药高等专科学校， 贵州 遵义563002； 3．六盘水市人民医院 病理科， 贵州 六盘水553001)

目的： 探讨亚慢性砷中毒对大鼠睾丸重量及组织形态的影响。方法: 清洁级SD雄性大鼠40只，体重100～120 g，随机分为对照组、砷低剂量组、砷中剂量组和砷高剂量组4组，每组10只，分笼喂养；对照组自由饮蒸馏水，染砷组自由饮用含三氧化二砷(As203)的蒸馏水，砷浓度分别是25、50和100 mg/L；连续喂养3个月后处死大鼠，取大鼠双侧睾丸称重并制作睾丸组织切片HE染色，采用光镜观察睾丸组织的形态改变。结果： 砷中、高剂量组的睾丸重量明显降低，与对照组比较，差异具有统计学意义(P<0.05)；与对照组比较，染砷组大鼠睾丸石蜡切片HE染色显示染毒组睾丸组织形态发生变化，睾丸生精小管间隙增宽，生精上皮结构疏松，层次排列紊乱并且层次逐渐减少，精原细胞出现空泡样改变，管腔中可见脱落的生精细胞，睾丸间质充血、渗出，尤其砷中、高剂量组较明显。结论： 亚慢性砷中毒对睾丸组织有一定的损伤作用，这种损害作用与砷剂量可能有关。

砷中毒； 睾丸； 大鼠，Sprague-Dawley； 生精上皮； 精原细胞

[Abstract]Objective: To investigate the effect of the sub-chronic arsenic poisoning on the testis weight and tissue morphology of rats. Method: 40 Sprague-Dawley (SD) rats in cleanliness grade, weighing 100 g～200 g each, were divided randomly into four groups: control group, low dose group, middle dose group and high dose group, 10 in each group, and separated and fed respectively. The rats infected with sub-chronic arsenic poisoning are treated with AS2O3 dissolved in distilled water. The arsenic concentration in low, middle and high dose group is 25 mg/L, 50 mg/L, and 100 mg/L, respectively. The rats in control group drink distilled water freely. The feeding continuously last three months. At the end of the experiment, all the rats were killed. The testis of both sides were weighed and testis tissue were stained with HE staining. The pathologic changes of testicle tissues were observed by light microscope. Results: The testis weight of the middle and high dose group was significantly decreased compared with that of control group, and difference was statistically significant(P<0.05). Compared with control group, HE staining showed that morphological changes were observed in testis of the arsenic poisoning groups, such as the gap widening in the seminiferous tubule of testis, the loose structures of the spermatogenic epithelium, disorder of arrangements of layers and gradual reductions of layers, vacuolar changes in the spermatogonium, spermatogenic cells in the lumen, hyperemia and exudations in the interstitial testis, especially in the middle and high dose groups. Conclusion: Chronic arsenic poisoning has a certain damage to testicular tissue, which may be associated with the dosage of arsenic.

[Key words]arsenic poisoning; testis; rats，Sprague-Dawley； spermatogenic epithelium； spermatogonium

我国砷储量居世界第一，也是受慢性砷中毒危害较严重的国家。砷广泛存在于水源、土壤和空气中，长期摄入过量砷(>0.05 mg/L)可引起砷中毒[1-2]。人体通过消化道或呼吸道等长期、 过量地摄入砷化合物，可引起慢性砷中毒[3]。有研究表明超过生理需要量的砷对生殖功能有一定的影响，但对睾丸组织形态的影响国内外报道较少。病理组织学检查是亚慢性毒性试验中最重要的检测指标之一，目的是确定化学毒物对机体毒作用的靶部位、损害的性质和程度，从病理学角度寻找化学毒物与病理改变的剂量效应关系，为了解化学毒物的毒效应及其机制提供依据[4]。为……