刘 伟郭思佳郝正华封继宏孙增涛△（.天津中医药大学第二附属医院，天津 30093；.天津中医药大学研究生院，天津 30093）

·研究报告·

清喉利咽颗粒联合养阴清肺糖浆止咳作用机制研究*

刘 伟1郭思佳1郝正华2封继宏1孙增涛1△
（1.天津中医药大学第二附属医院，天津 300193；2.天津中医药大学研究生院，天津 300193）

目的 探讨清喉利咽颗粒联合养阴清肺糖浆的止咳作用机制。方法 将实验SD大鼠随机等分为6组，即对照组、模型组、甘草合剂组、养阴清肺糖浆组、清喉利咽颗粒组、联合组，除对照组外均造感染后咳嗽模型，造模成功后给予相应药物灌胃，空白组给予等体积0.9%氯化钠注射液灌胃。检测相关指标：通过氨水诱咳测大鼠咳嗽潜伏时间和3 min内咳嗽次数；通过HE染色法观察肺组织病理改变；收集支气管肺泡灌洗液（BALF），采用ELISA法检测BALF中细胞因子［白介素-4（IL-4）、白介素-8（IL-8）、肿瘤坏死因子-α （TNF-α）］、神经肽P物质（SP）及降钙素基因相关肽（CGRP）的含量。结果 甘草合剂组、养阴清肺糖浆组、清喉利咽颗粒组以及联合组大鼠咳嗽次数均明显减少，BALF中SP，CGRP，IL-4，IL-8，TNF-α水平显著降低（P＜0.05），其作用效果由高到低依次是：养阴清肺糖浆联合清喉利咽颗粒、清喉利咽颗粒、养阴肺糖浆、甘草合剂。结论 养阴清肺糖浆联合清喉利咽颗粒能够明显抑制感染后咳嗽，其机制与减轻肺组织局部炎症反应有关。

感染后咳嗽 清喉利咽颗粒 养阴清肺糖浆

【Abstract】Objective：To study the mechanism of the effects of Qinghou Liyan granules with Yangyin Qingfei syrup on relieving a cough.Methods：Experimental SD rats were randomly divided into 6 groups，the control group，the model group，Gancao group，Yangyin Qingfei group，Qinghou Liyan group，and the combination group.All the other groups，except the control group，were made cough infection model.After the model was successfully made，the corresponding drugs were administered to the stomach.The control group was given equal volume of normal saline，once per day for 15 days.The following indicators were detected：Ammonia induced cough latent period and cough within 3 minutes，by HE staining to observe the lung pathological changes，collecting bronchoalveolar lavage fluid（BALF），ELISA method to detect in the BALF cell factor（IL-4，IL-8，TNF-α），neuropeptide substance P（SP）and Calcitonin gene-related peptide（CGRP）.Results：Gancao group，Yangyin Qingfei group，Qinghou Liyan group，the combination group can reduce cough times in rats.They can reduce the levels of SP，CGRP，IL-4，IL-8，TNF-αin the BALF.Its effect from high to low in turn is Qinhou Liyan granules with Yangyin Qingfei syrup，Qinghou Liyan granules，Yangyin Qingfei syrup，Gancao syrup.Conclusion：Yangyin Qingfei syrup and Qinghou Liyan granules can relieve cough caused by infection.The mechanism is related to the reduction of local inflammatory response in lung tissue.

【Key words】Post-infectious cough；Qinghou Liyan granules；Yangyin Qingfei syrup

清喉利咽颗粒来源于清代宫廷秘方 “宽膈利咽代茶饮”，原为御用清喉利咽之上品。养阴清肺糖浆是由治喉名方养阴清肺汤化裁而来，记载于清代名医郑梅涧的喉科专著《重楼玉钥》中。……