沈俊逸 方邦江凌 丽 陈振翼 王晓翠（上海中医药大学附属龙华医院，上海 200032）

·研究报告·

复元醒脑汤对糖尿病脑梗死大鼠脑组织SDF-1、CXCR4、VEGF基因及蛋白表达作用的研究*

沈俊逸 方邦江△凌 丽 陈振翼 王晓翠
（上海中医药大学附属龙华医院，上海 200032）

目的 观察复元醒脑汤对糖尿病脑梗死大鼠脑组织SDF-1、CXCR4、VEGF基因及蛋白表达的干预作用。方法 120只健康雄性SD大鼠随机分为正常对照组、假手术组、模型组、治疗组各30只。造模完成后，治疗组予以复元醒脑汤，假手术组、模型组予以0.9%氯化钠注射液，灌胃7 d。测定比较各组实验大鼠脑组织SDF-1、CXCR4、VEGF的mRNA及蛋白表达水平。结果 各组脑组织SDF-1、CXCR4、VEGF三种基因及蛋白表达具有类似趋势，由高到低依次为正常对照组、治疗组、模型组及假手术组。正常对照组高于假手术组；治疗组高于模型组（均P＜0.01）。结论 复元醒脑汤可上调SDF-1、CXCR4及VEGF的mRNA及蛋白表达水平，改善受损血管内皮功能及修复功能而促进局部血管再生和侧支循环建立。

糖尿病脑梗死 复元醒脑汤 SDF-1 CXCR4 VEGF

【Abstract】Objective：To carry out the effect of Fuyuan Xingnao Decoction on the gene expression and protein secretion of SDF-1、CXCR4 and VEGF in diabetic cerebral infarction rats.Methods：Randomly all the enrolled healthy male SD rats were divided into 4 groups as Group C（control），Group AD（treated with placebo after acute cerebral infarction combined with diabetes mellitus），Group DM（diabetes mellitus without acute cerebral infarction）and Group AT （treated with Fuyuan Xingnao Decoction after acute cerebral infarction combined with diabetes mellitus）.After 7 day，the gene expression and protein secretion of SDF-1、CXCR4 and VEGF were measured in each samples.Results：Descendingly among all the groups the gene expression and protein secretion order of SDF-1，CXCR4 and VEGF were Group C，Group AT，Group AD and Group DM.Those of Group C were significantly overtopped than those of Group DM （P＜0.01）.Those of Group AT were significantly elevated in comparison with Group AD（P＜0.01）.Conclusion：Fuyuan Xingnao decoction can improve the gene expression and protein secretion of SDF-1、CXCR4 and VEGF in diabetic cerebral infarction rats，and then improve the vascular regeneration and the establishment of the collateral circulation.

【Key words】Fuyuan Xingnao Decoction；Diabetic cerebral infarction；SDF-1；CXCR4；VEGF

众多研究表明糖尿病患者脑梗死发病率、致残率及死亡率均较非糖尿病者高，机制尚不明确［1-7］。这给临床治疗及预防带来很大困难。本实验所用方药复元醒脑汤是方邦江教授临床常用的治疗糖尿病并发脑梗死基础方，经临床验证疗效显著。前期多项研究业已证实，复元醒脑汤能改善糖尿病脑梗死大鼠神经行为和缺血脑组织病理结构，降低脑系数、脑组织含水量、脑血管通透性而缓解脑水肿［8-10］。研究结果示，内……