胃癌发生过程中Survivin促血管生成作用的研究

2016-09-09潘存伟林瑶光

现代消化及介入诊疗 2016年2期

潘存伟　林瑶光

潘存伟林瑶光

目的探讨胃癌发生过程中Survivin促进血管生成的作用。方法用免疫组化方法检测慢性非萎缩性胃炎、胃癌癌前病变、胃癌组织中Survivin的表达及MVD情况，分析Survivin的表达与胃癌临床病理参数之间的关系，分析Survivin的表达与MVD的关系。结果Survivin蛋白在胃癌组织中的表达率为71.67%，显著高于慢性非萎缩性胃炎的3.33%及胃癌前病变组织的30.00%，三组间差异有统计学意义（P<0.05）；Survivin蛋白表达与肿瘤分化程度、胃癌局部浸润、淋巴结转移和TNM分期有关（P< 0.05）。Survivin表达阳性的胃癌组织及癌旁组织中MVD表达高于Survivin表达阴性的胃癌组织及癌旁组织（P<0.05）。结论Survivin的过度表达是胃癌发生过程中的早期事件，可能可以促进胃癌血管形成。

胃癌；Survivin；微血管密度；癌前病变

【Abstract】Objective To explore the expression of Survivin and its relationship with tumor angiogenesis in gastric cancer.Method The expression level of Survivin and microvessel density(MVD)in chronic non-atrophic gastritis,precancerous and cancer tissues were detected by immunohistochemistry.The relationship between Survivin and clinicopathological parameters of gastric cancer and the relationship between Survivin and MVD were also analyzed.Results The expression level of Survivin in gastric cancer tissue was 71.67%which was significantly higher than that in chronic non-atrophic gastritis(3.33%)and precancerou tissue(30.00%)(P <0.05).The expression levels of survivin were correlated with degree of tumor differentiation,regional lymph node metastasis,TNM staging and serosal invasion(P<0.05).The MVD in Survivin-positive carcinoma and carcinoma-adjacent tissues were higher than those in Survivin-negative tissues(P<0.05).Conclusion The overexpression of Survivin is an early event in the development of gastric cancer and likely involved in the aniogenesis of gastric cancer.

【Key words】Gastric cancer;Survivin;Microvessel density;Precancerous lesions

肿瘤的生长、浸润是肿瘤转移的前提，肿瘤新生血管的形成是其中的重要原因，如果没有血管生成，原发肿瘤的生长不会超过2 mm3。Survivin是凋亡抑制蛋白家族的成员之一，可以调节细胞周期，在细胞周期的G2/M期特异性表达[1]，可以与细胞凋亡关键蛋白caspase-3和caspase-7结合，抑制其激活从而抑制细胞凋亡[2]。近年来发现Survivin与血管形成有关[3]，随着其表达量的增加，微血管密度（Microvessel density，MVD）可能呈上升的情况，但在胃癌中的相关研究较少。本研究通过检测慢性非萎缩性胃炎、癌前病变、胃癌组织中Survivin的表达量及与微血管密度的关系，探讨Survivin促血管生成在胃癌发生过程中的作用，为胃癌的治疗寻找新的方向。

资料与方法