吴华涛，沈家欣，刘　静，

Notch3增强三阴性乳腺癌MDAMB-231细胞对顺铂的敏感性

吴华涛1，沈家欣2，3，刘静2，3，*

（1. 汕 头大学医学院第一附属医院普通外科，广东汕头515041；2. 汕 头大学医学院长江学者实验室，广东汕头515041；3. 广 东省乳腺癌诊治研究重点实验室，广东汕头515041）

目的： 探讨干细胞相关因子Notch3在三阴性乳腺癌MDA-MB-231细胞对顺铂化疗敏感性中的作用。方法：用Lipofectamine 2000试剂转染乳腺癌MDA-MB-231细胞并用G418筛选稳定转染细胞系，根据转染质粒不同分为MDA-MB-231-PCLE组(对照组)和MDA-MB-231-N3ICD组(过表达Notch3组)，应用实时荧光定量PCR和Western blot方法检测不同组间Notch3表达的差异；用CCK8法检测各组MDA-MB-231细胞对顺铂敏感性的差异；同时利用Lipofectamine 2000转染Notch3的小干扰序列，根据转染序列不同分为T47D-NC(对照组)和T47D-siNotch3(Notch3敲减组)；采用免疫印迹检测Notch3表达改变后凋亡相关因子caspase-3的表达情况。结果：与对照组比较，高表达Notch3后三阴性乳腺癌对顺铂的敏感性增加(P＜0.05)，同时caspase-3的表达随着Notch3的增加而增加，而在高表达Notch3的乳腺癌T47D细胞中，敲减Notch3的表达使caspase-3表达减少。结论：高表达Notch3可以增加三阴性乳腺癌MDA-MB-231细胞对顺铂的敏感性，从而增强患者化疗疗效。

Notch3；顺铂；乳腺癌；化疗

【ABSTRACT】OBJECTIVE: To investigate the effect of stem cell-related factor，Notch3 on human triple negativebreast cancer cell，MDA-MB-231 and its resistance to cisplatin，and explore if overexpressed Notch3 can enhance thesensitivity of resistance cells to cisplatin. METHODS：Lipofectamine 2000 was applied to transfect Notch3 plasmid intoMDA-MB-231. These cells were divided into MDA-MB-231-PCLE group and MDA-MB-231-N3ICD group，accordingto the use of different plasmids. RT-PCR and Western blot methods were used to analyze the expression of Notch3 indifferent groups. The effects of cisplatin on cell proliferation were assessed by CCK8 assay and evaluated by GraphPadPrism 5. Lipofectamine 2000 was also applied to transfect the siNotch3 or scramble to cells and they were divided intoT47D-NC and T47D-siNotch3 groups. The protein levels of apoptosis-related factor were detected using RT-PCRmethods and Western blot. RESULTS：Then efficacy of cisplatin to triple negative breast cancer cells was enhanced byoverexpression of Notch3. Apoptosis-related factor，caspase-3 was up-regulated by Notch3 overexpression. However，siNotch3 induced the downregulation of caspase-3 in T47D，in which the expression of Notch3 was high.CONCLUSION：Overexpression of Notch3 enhanced the sensitivity of MDA-MB-231 to cisplatin which suggests thatthis m ay improve the efficiency of chemotherapy in patients with breast cancer.

【KEY WORDS】Notch3；cisplatin；breast cancer；chemotherapy

乳腺癌现已成为女性最常见的恶性肿瘤，占女性恶性肿瘤新发病例的29%[1]。根据肿瘤细胞雌激素受体(estrogen receptor，ER)、孕激素受体(progesterone receptor， PR)和人表皮生长因子受体(human epithelialgrowth factor receptor 2，HER2)表达的不同可将乳腺癌分为不同的分子亚型，其中ER/PR/HER2均为阴性的三阴性乳腺癌(triple-negative breast cancer，TNBC)是一种特殊类型的乳腺癌，复发和转移率高，预后较差。由于缺乏内分泌和抗HER2靶向治疗的靶点，TNBC的内科治疗以化疗为主，但与其他亚型乳腺癌相比，TNBC具有更强的耐药性。……