黎秀平　李海燕　谢健鸣

[摘要]目的 考察石墨原子吸收法测定丹参中铅、镉重金属含量的可行性。 方法 建立原子吸收法测定丹参中Pb、Cd两种重金属的含量，Cd的灰化温度为950℃，原子化温度1600℃， Pb的灰化温度为800℃，原子化温度1700℃，将丹参药材经微波消解处理后，采用上述方法3批次样品中铅、镉含量。 结果 经方法学检查，Cd、Pb均符合相关标准，Pb在0.0～50.0μg/L浓度范围内，与吸光度呈良好线性相关，相应回归方程是：y=235.5A1-254.3（r=0.9995），Cd在0.0～5.0μg/L浓度范围内，与吸光度呈良好线性相关，相应回归方程是：y=432.1A2-53.4（r=0.9996），所测各批次丹参药材的铅、镉重金属含量均合格。 结论 采用原子吸收光谱法测定丹参药材Cd、Pb重金属含量，操作快捷、方法可靠、精密度和稳定性高，可用于丹参重金属测定。

[关键词]原子吸收分光光度法；丹参；重金属；微波

[中图分类号] R286 [文献标识码] A [文章编号] 2095-0616（2016）09-50-03

[Abstract]Objective To investigate the feasibility of determination of two kinds of heavy metals of Pb and Cd in Salvia miltiorrhiza by graphite atomic absorption spectrometry. Methods The atomic absorption method was established to be used for the determination of two kinds of heavy metals of Pb and Cd in Salvia miltiorrhiza. The ashing temperature of Cd is 950℃， and the atomization temperature of Cd is 1600℃. The ashing temperature of Pb is 800℃， and the atomization temperature of Pb is 1700℃. After microwave digestion of Salvia， Pb and Cd in 3 batches of samples were determined by atomic absorption spectrometry. Results Through the methodology， Pb and Cd were in line with the relevant standards. In the concentration range of 0 to 50 g/L， Pb had a good linear relationship with absorbance. The corresponding regression equation was y=235.5A1-254.3（r=0.9995）. In the concentration range of 0.0 to 5.0μg/L， Cd had a good linear relationship with absorbance. The corresponding regression equation was y=432.1A2-53.4（r=0.9996）. The contents of lead and cadmium in all the batches of Salvia miltiorrhiza were qualified. Conclusion Atomic absorption spectrometry is fast， reliable， accurate and stable to be used for the determination of Cd and Pb in Salvia miltiorrhiza. Atomic absorption spectrometry can be used for the determination of heavy metals in Radix Salvia miltiorrhiza.

[Key words] Atomic absorption spectrometry； Salvia； Heavy metal； Microwave

丹参（Salvia miltiorrhiza Bge），为唇形科植物鼠尾草属的干燥根及根茎[1]，味苦，性微寒，是较为常见的中药材之一。丹参因其根赤肉紫，形状似参，故得名，主产于河南、四川等地[2]，具有养血安神、调经止痛、活血祛瘀等功效[3]。丹参化学成分分水溶性和脂溶性两种[4]，前者以酚酸为主，如：丹参酸甲、乙、丹参素等，有抗肝纤维化、治疗消化溃疡、改善心血管等诸多疗效，后者为丹参酮类为主，如：异丹参酮Ⅰ、异隐丹参酮Ⅱ、丹参酮ⅡA等[5]。随着目前丹参类药物：复方丹参滴丸、丹参片等大范围使用，目前市售丹参需求量较大。由于目前环境污染日益严重，土壤问题等带来的中药重金属含量不达标问题，对中药材质量和药效产生影响，为检验工作中的重点之一。……