方　谦，穆　玉，周　雪，瞿亚男，彭　伟

华北理工大学口腔医学院 唐山 063000

△女，1988年11月生，硕士，医师，研究方向：口腔内科学，E-mail:416385376@qq.com



生物活性玻璃对体外脱矿釉质再矿化的影响

方谦△，穆玉，周雪，瞿亚男，彭伟

华北理工大学口腔医学院 唐山 063000

△女，1988年11月生，硕士，医师，研究方向：口腔内科学，E-mail:416385376@qq.com

关键词生物活性玻璃；再矿化；显微硬度；脱矿釉质

摘要目的：观察生物活性玻璃对早期脱矿釉质再矿化的作用。方法：将40块牛牙釉质块建立人工龋模型后随机分为4组：生物活性玻璃组(用质量分数6%生物活性玻璃进行再矿化处理)、GC护牙素组、NaF组(用质量分数2%NaF进行再矿化处理)和去离子水组。采用pH循环法进行再矿化处理，2次/d，5 min/次，循环15 d。用显微硬度仪测量脱矿前、再矿化前及再矿化后牙釉质表面的显微硬度，荧光显微镜观察早期釉质龋表层下的荧光带厚度，测定脱矿深度。结果：生物活性玻璃组、GC护牙素组、NaF组再矿化后显微硬度均较再矿化前增加，且生物活性玻璃组提高幅度最大(P<0.05)。4组再矿化区荧光带厚度均较脱矿区降低(P<0.05)，其中生物活性玻璃组、GC护牙素组、NaF组均大于去离子水组(P<0.05)。结论：质量分数6%生物活性玻璃溶液促进脱矿釉质再矿化的疗效较好。

Effect of bioactive glass on demineralization enamel remineralization in vitro

FANGQian,MUYu,ZHOUXue,QUYanan,PENGWei

CollegeofStomatology,NorthChinaUniversityofScienceandTechnology,Tangshan063000

Key wordsbioactive glass;remineralization;microhardness；demineralization enamel

AbstractAim: To observe the effect of bioactive glass on the remineralization of demineralization enamel. Methods: Forty bovine teeth were subjected to establish demineralization enamel model, and then were allocated into four groups randomly(10 in each group) and treated with 6% bioactive glass, casein phosphopeptide amorphous calcium phosphate(CPP-ACP),2% sodium fluoride(NaF) and deionized water,respectively.Then they were subjected to the pH-cycling,two times a day and 5 minutes each time, cycling for 15 days for remineralization. The surface microhardness(SMH)of the enamel before demineralization,before and after remineralization were measured by microhardness detector. Thickness of fluorescence beneath the surface of early enamel caries in the demineralization area and the remineralization area were detected by fluorescence microscopy.Results: The SMH after remineralization in bioactive glass,CPP-ACP and NaF groups was higher than those before remineralization, and the change of bioactive glass group was the highest(P<0.05). The thickness of fluorescence in the remineralization area was lower than that in the demineralization area in the four groups(P<0.05), and the demineralization depth of bioactive glass,CPP-ACP and NaF groups was higher than the deionized water group(P<0.05).Conclusion: 6% bioactive glass solution had better effect on remineralization of demineralization enamel.

早期釉质龋是指釉质表面病变区失去光泽，呈片块状白垩色改变而无实质性缺损。它的治疗一般采取非手术治疗，即采用药物或再矿化等保守方法使龋病病变终止或消除。常用的材料有氟化物、酪蛋白磷酸肽-无定形磷酸钙(CPP-ACP)、生物活性玻璃及渗透树脂等，其中氟化物的使用占主导地位，但其安全性仍存在一定的争议[1]。作者前期已对生物活性玻璃促进早期釉质龋再矿化的最适浓度进行了研究，发现质量分数6%的生物活性玻璃溶液再矿化效果较好[2-3]。……