丁文清

[摘要] 目的 探讨高迁移率族蛋白B1（HMGB1）和增殖细胞核抗原（PCNA）在宫颈癌组织中的表达及其相关因素。 方法 选取2008年9月～2014年10月我院收治的60例宫颈癌患者作为研究对象，手术留取宫颈癌组织标本60份（宫颈癌组）、癌旁组织标本60份（癌旁组）、正常组织标本60份（正常组），采用免疫组织化学法检测三组HMGB1和PCNA的表达，并分析两指标与临床病理特征的关系。 结果 宫颈癌组、癌旁组和正常组的HMGB1阳性表达率分别为96.7%、23.3%和6.7%，差异有统计学意义（P<0.05）；宫颈癌组、癌旁组和正常组的PCNA阳性表达率分别为91.7%、66.7%和43.3%，差异有统计学意义（P<0.05）。HMGB1的表达与TNM分期、淋巴结转移及肿瘤分化程度相关，PCNA的表达与TNM分期、淋巴结转移、浸润深度及肿瘤分化程度相关，差异有统计学意义（P<0.05）。 结论 宫颈癌患者的宫颈癌组织中，HMGB1及PCNA表达水平均显著增高，两项指标对宫颈癌的预后评估具有重要意义。

[关键词] 宫颈癌；高迁移率族蛋白B1；增殖细胞核抗原

[中图分类号] R711.74 [文献标识码] A [文章编号] 1674-4721（2016）02（b）-0050-03

Expression of high mobility group protein B1 and proliferating cell nuclear antigen in cervical cancer tissues and and the analysis of related factors

DING Wen-qing

Department of Gynaecology，Maternal and Child Health Hospital of Longgang District in Shenzhen City，Shenzhen 518172，China

[Abstract] Objective To investigate the expression of high mobility group protein B1 （HMGB1） and proliferating cell nuclear antigen （PCNA） in cervical cancer tissues and its related factors. Methods 60 cases of cervical cancer treated in our hospital from September 2008 to October 2014 were selected as the research objects，and 60 cases of cervical cancer tissues（cervical cancer group），60 cases of adjacent tissue samples （adjacent group） and 60 cases of normal tissue samples （normal group） were collected by surgery.The expression of HMGB1and PCNA among three groups were detected by immunohistochemistry，and the relationship between the two indexes and clinical pathological features were analyzed. Results The positive expression rates of HMGB1 in cervical cancer group，adjacent group and normal group was respectively 96.7%，23.3% and 6.7%，the difference was statistically significant（P<0.05）.The positive expression rates of PCNA in cervical cancer group，adjacent group and normal group was respectively 91.7%， 66.7% and 43.3%，the difference was statistically significant（P<0.05）. The expression of HMGB1 was related to TNM stage，lymph node metastasis and degree of tumor differentiation.The expression of PCNA was related to TNM stage，lymph node metastasis，invasion depth and degree of tumor differentiation.The differences were significant（P<0.05）. Conclusion The expression level of HMGB1 and PCNA are significantly increased in cervical cancer tissues of patients with cervical cancer，and the two index have important significance to the prognosis of cervical cancer.

[Key words] Cervical cancer；High mobility group protein B1；Proliferating cell nuclear antigen

宫颈癌是一种女性生殖系统恶性肿瘤，占女性恶性肿瘤的第二位[1]，全球每年约有28万死亡病例产生[2]，是女性常见的死因之一。高迁移率族蛋白B1（HMGB1）是一种低分子非组蛋白，其基因具有参与神经生长、细胞增殖、迁移、分化、炎症反应等功能。在多种恶性肿瘤中，均存在HMGB1的过表达现象，影响肿瘤的恶性程度及预后[3]。增殖细胞核抗原（PCNA）是一种细胞核蛋白，在一定程度上可判断细胞增殖活性，反映细胞的增殖状态[4]。本研究探讨HMGB1及PCNA在宫颈癌患者宫颈癌组织中的表达水平变化，并对其临床意义进行研究，旨在为宫颈癌的治疗及预后提供帮助。

1 资料与方法

1.1 一般资料

选取2008年9月～2014年10月我院收治的60例宫颈癌患者作为研究对象，手术留取宫颈癌组织标本60份（宫颈癌组）及癌旁组织标本60份（癌旁组），另取正常宫颈组织标本60份作为正常组。患者年龄21～75岁，平均（43.2±12.7）岁，术前均未接受放疗、化疗等针对肿瘤的治疗；……