冯 慧骆善伟杜 艳余丽霞穆金虎陈玉泽,3李文建周利斌

1（中国科学院近代物理研究所 兰州 730000）2（中国科学院大学 北京 100049）3（甘肃农业大学 兰州 730070）



氩离子束辐照拟南芥干种子的生物学效应

冯 慧1,2骆善伟1,2杜 艳1余丽霞1,2穆金虎1,2陈玉泽1,3李文建1周利斌1

1（中国科学院近代物理研究所 兰州 730000）
2（中国科学院大学 北京 100049）
3（甘肃农业大学 兰州 730070）

摘要以拟南芥（Columbia生态型）为材料，利用氩离子束辐照干种子，通过对存活率、根长、下胚轴长和株高的测定来研究不同剂量的氩离子束辐照对拟南芥的生物学效应。结果显示，随着吸收剂量的增大，拟南芥的根长、下胚轴长和株高都呈现出下降趋势；但存活率在低剂量辐照时无显著变化，高剂量辐照时逐渐降低。运用随机扩增多态性DNA (Random amplified polymorphic DNA, RAPD)技术对氩离子辐照后拟南芥的DNA指纹图谱进行分析，结果表明，各个处理组和对照组之间的相似性系数在0.556～0.944之间，并且相似性系数与剂量之间没有显著相关性。对照组与处理组之间的差异体现为扩增条带数目的减少和增多以及条带亮度的不同。综合分析表明，氩离子束辐照拟南芥干种子产生显著的当代损伤效应，较高剂量下会抑制生长发育，同时引起基因组DNA一定程度的多态性变化。

关键词氩离子束，辐照，拟南芥，生物学效应，随机扩增多态性DNA

Supported by the National Natural Science Foundation of China (11205218, 11275171, 11405234), CAS "Light of West China"

Program (29Y506020) and Youth Innovation Promotion Association of CAS (29Y506030)

First author: FENG Hui, female, was born in February 1992 and graduated from Northwest Normal University in June 2014. Now she is a master candidate of Institute of Modern Physics, Chinese Academy of Sciences, majoring in bioengineering and focusing on basic study on mutation breeding. E-mail: fenghui@impcas.ac.cn

Received 15 September 2015; accepted 13 October 2015

Biological effects of argon ion beams on dry seeds of Arabidopsis thaliana

FENG Hui1,2LUO Shanwei1,2DU Yan1YU Lixia1,2MU Jinhu1,2CHEN Yuze1,3LI Wenjian1ZHOU Libin1

1(Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China)
2(University of Chinese Academy of Sciences, Beijing 100049, China)
3(Gansu Agricultural University, Lanzhou 730070, China)

ABSTRACT To study the biological effects of argon ion beams provided by Heavy Ion Medical Accelerator in Chiba (HIMAC, Program No.13J404) in National Institute of Radiological Sciences (NIRS) on Arabidopsis thaliana, the postembryonic development of irradiated dry seeds, such as survival rate, root length, hypocotyls length, plantheight and bolting rate, were investigated. The results showed that survival rate, root length, hypocotyls length, plant height and bolting rate decreased with the increasing doses. The variations of genomic DNA fingerprints of the irradiated samples were analyzed by using random amplified polymorphic DNA (RAPD) technique. RAPD results demonstrated that the genetic similarity coefficient between the controls and treatment groups were 0.556～0.944. But the similarity coefficients among treatment groups irradiated with different dosages had no obvious correlations. All the results indicate that M1 seedlings of Arabidopsis thaliana irradiated by argon ions has obvious biological effects on both the plant developments and genomic DNA variations.

KEYWORDS Argon ion beams, Irradiation, Arabidopsis thaliana, Biological effects, Random amplified polymorphic DNA (RAPD)

CLC Q691.5, TL99

拟南芥(Arabidopsis thaliana)属于十字花科，是植物科学中一种重要的模式植物，在生物学研究中具有以下一些优势[1-2]：(1)生命周期很短，仅为60 d左右，并可通过改变光照时间和光照强度等培养条件来改变生命周期；……