姚丽娟，罗以勤，孙安源，卜国平，王力(安徽医科大学附属省立医院，合肥230001)

用于检测胃蛋白酶原、胃泌素-17的血浆样本保存条件探讨

姚丽娟，罗以勤，孙安源，卜国平，王力
(安徽医科大学附属省立医院，合肥230001)

摘要:目的探讨用于检测胃蛋白酶原( PG)、胃泌素-17( G-17)的血浆样本的保存条件。方法血浆样本180例份，采用ELISA双抗体夹心法定量检测血浆PG-Ⅰ、PG-Ⅱ、G-17，作为第1天结果(对照)。将剩余血浆样本随机分为1、2组，每组90份;每组又分为血浆未分离组(离心后未分离血浆)、血浆分离组(离心后取血浆)和稳定剂组(离心后取血浆，并加入稳定剂)，每组各30份。将各组每个样本平均分装6管后分别置于室温、4℃和－20℃环境下保存，每天固定时间取1组样本检测PG-Ⅰ、PG-Ⅱ，取2组样本检测G-17，均连续检测7 d(包括第1天)。将室温、4℃、－20℃温度下保存不同时间点时血浆PG-Ⅰ、PG-Ⅱ、G-17浓度与其保存第1天时浓度比较，将P＞0.05的天数作为稳定性维续时间。结果血浆未分离组室温下PG-Ⅰ稳定性维持2 d，PG-Ⅱ为6 d，G-17为1 d; 4℃时分别为5、7、3 d;－20℃时分别为4、3、2 d。血浆分离组室温下PG-Ⅰ稳定性维持7 d，PG-Ⅱ为6 d，G-17为2 d; 4℃时分别为5、7、5 d;－20℃时分别为5、7、6 d。稳定剂组室温下PG-Ⅰ稳定性维持7 d，PG-Ⅱ为7 d，G-17为7 d; 4℃时分别为5、6、4 d;－20℃时分别为7、5、4 d。结论在临床标本7 d保存期内，PG和G-17检测样本推荐使用血浆分离后低温保存，如使用稳定剂，推荐血浆分离后室温保存。

关键词:血浆;胃蛋白酶原;胃泌素-17;室温;稳定剂

Storage conditions for plasma samples of pepsinogen and gastrin-17

YAO Li-juan，LUO Yi-qin，SUN An-yuan，BU Guo-ping，WANG Li
( The Affiliated Provincial Hospital of Anhui Medical University，Hefei 230001，China)

Abstract:Objective To investigate the storage conditions for plasma samples of pepsinogen ( PG) and gastrin-17 ( G-17).Methods A total of 180 plasma samples were collected to detect PG-Ⅰ，PG-Ⅱand G-17 as the first day's consequence ( the control group) by ELISA double antibody sandwich method.The remaining samples were divided into group 1 and group 2 with 90 samples in each.Each group was equally divided into the non-separated group with upper layer which was plasma after centrifugation，the separated group with only plasma after centrifugation and the stabilizer group with plasma and stabilizer，30 cases in each group.Every sample in each group was packaged into 6 pipes which were separately stored at room temperature，4℃and－20℃，and we took the samples from group 1 to detect PG-Ⅰand PG-Ⅱ，or G-17 in the group 2 at the same time for 7 days.The concentrations of PG-Ⅰ，PG-Ⅱand G-17 from non-separated，separated and stabilizer groups in the different storage temperatures at different time were compared with the consequences of the first day，and the days of P＞0.05 was taken as the maintain time of stability.Results In the non-separated group，the plasma PG-Ⅰ，PG-Ⅱand G-17 maintained the levels for 2 days，7 days and 1 day at room temperature; 5 days，7days and 3 days at 4℃; and 4 days，3 days and 2 days at－20℃.In the separated group，the plasma PG-Ⅰ，PG-Ⅱand G-17 could maintain the levels for 7 days，6 days and 2 days at room temperature; 5 days，7days and 5 days at 4℃; and 5 days，7 days and 6 days at－20℃.The plasma PG-Ⅰ，PG-Ⅱand G-17 in the stabilizer group could maintain the levels for 7 days，7 days and 7 days at room temperature; 5 days，6 days and 4 days at 4℃; and 7 days，5 days and 4 days at －20℃.Conclusion In 7 days of storage for clinic detection，PG and G-17 detection samples are recommended to storage in low temperature after centrifugation，and are recommended to preserve at room temperature if we add stabilizer.

Key words:plasma; pepsinogen; gastrin-17; room temperature; stabilizer

胃蛋白酶原( PG)和胃泌素-17( G-17)在血中浓度的变化可以准确反映胃黏膜消化腺分泌功能的改变，并与胃黏膜组织学变化和病变程度高度相关［1～4］，有望成为胃癌的早期诊断标志物。……