miR-17-5p在宫颈癌组织中的表达变化及其对宫颈癌细胞生长增殖影响

魏倩，张鹏

(天津医科大学肿瘤医院暨国家肿瘤临床医学研究中心及天津市肿瘤防治重点实验室,天津300060)

摘要：目的观察miR-17-5p在宫颈癌组织中的表达变化及其对宫颈癌细胞生长和增殖的影响。方法用实时荧光定量PCR法对20例宫颈癌患者的癌组织和癌旁组织中的miR-17-5p进行检测。将人宫颈癌细胞株HeLa分组，分别转染人工构建的miR-17-5p质粒、阴性对照质粒、miR-17-5p的反义寡核苷酸质粒及无关序列寡核苷酸质粒，采用MTT法和平板克隆形成实验检测细胞生长活性和克隆数目。结果宫颈癌组织、癌旁组织中miR-17-5p的相对表达量分别为0.315±0.185、1.143±1.373，两者相比，P<0.05。与转染阴性对照质粒的HeLa细胞相比，转染miR-17-5p质粒的HeLa细胞生长活性、克隆形成率明显降低(P均<0.05)；与转染无关序列寡核苷酸质粒的HeLa细胞相比，转染miR-17-5p的反义寡核苷酸质粒的HeLa细胞生长活性、克隆形成率明显升高(P均<0.05)。结论 宫颈癌组织中miR-17-5p表达减少，其可抑制宫颈癌细胞的生长和增殖。

关键词：多顺反子miRNA基因；miR-17-5p基因；宫颈肿瘤；宫颈癌；宫颈癌细胞；细胞生长；细胞增殖

doi：10.3969/j.issn.1002-266X.2015.43.003

中图分类号：R737.33 文献标志码：A

基金项目：天津医科大学肿瘤医院科学研究基金资助项目(Y1207)。

作者简介：第一魏倩(1987-)，女，硕士研究生，检验技师，主要研究方向为分子生物学及临床检验诊断。E-mail： weiqian19870729@163.com

作者简介：通信张鹏(1956-)，男，教授，主任技师，主要研究方向为临床检验诊断。E-mail： laopang.56@163.com

收稿日期：(2015-07-06)

Expression changes of miR-17-5p in cervical cancer tissues and its

effects on cervical cancer cell growth and proliferation

WEIQian,ZHANGPeng

(TianjinMedicalUniversityCancerInstituteandHospital,NationalClinicalResearchCenterofCancer,

KeyLaboratoryofCancerPreventionandTherapy,Tianjin300060,China)

Abstract:ObjectiveTo observe the expression changes of miR-17-5p in the cervical cancer tissues and its effects on cervical cancer cell growth and proliferation. MethodsThe levels of miR-17-5p in cervical cancer tissues and adjacent tissues of 20 patients were detected by real-time quantitative PCR. We divided the human cervical cancer cell line HeLa into different groups, which were respectively transfected by artificial miR-17-5p plasmid, negative control plasmid, miR-17-5p antisense oligonucleotide (ASO) plasmid and irrelevant sequence oligonucleotide plasmid. MTT and colony experiment were used to detect the growth activity and clone number.ResultsThe relative expression of miR-17-5p in the cervical cancer tissues and adjacent tissues were 0.315±0.185 and 1.143±1.373 (P<0.05). The cell growth and cloning efficiency of HeLa cells transfected with pri-miR-17-5p were significantly decreased as compared with those of HeLa cells transfected by negative control plasmid (all P<0.05); the cell growth and cloning efficiency of HeLa cells transfected with pri-miR-17-5p ASO were significantly increased as compared with those of HeLa cells transfected by irrelevant sequence oligonucleotide plasmid (all P<0.05). Conclusion The expression of miR-17-5p was significantly down-regulated in the cervical cancer tissues, which inhibited the cell growth and proliferation of cervical cancer cells.

Key words: polycistronic mRNA; miR-17-5p gene; cervical neoplasms; cervical carcinoma; cervical carcinoma cells; cell growth; cell proliferation

miR-17-5p在不同肿瘤组织中的表达水平是不同的，在多种恶性实体瘤如肺癌、胃癌及肝癌等肿瘤中呈过表达[1～3]，而在乳腺癌和鼻咽癌等肿瘤中因该基因簇所在染色体区段的杂合性缺失导致其呈低表达[4]。上述研究结果表明miR-17-5p在不同肿瘤的发生发展过程中扮演了不同的生物学角色[5]。2014年1月，我们对miR-17-5p在宫颈癌组织中的表达变化及其对宫颈癌细胞生长和增殖的影响进行了观察。……