芝麻素对自发性高血压大鼠心肌氧化应激损伤的保护作用

李伟,杨解人,李文星,张俊秀,唐丽娟,杨慧,熊莺

(皖南医学院药理学教研室,安徽芜湖241002)

【摘要】目的：观察芝麻素对自发性高血压大鼠(spontaneously hypertensive rat,SHR)心肌iNOS和c-fos蛋白表达来探讨其可能保护机制。方法：SHR 35只，随机分为SHR模型组、芝麻素[40、80、160 mg/(kg·d)]组、卡托普利[30 mg/(kg·d)]组及WKY阴性组，每天灌胃给药1次，连续16周。末次给药后，称体质量，HE染色观察心肌病理变化；化学比色法测心肌组织中T-SOD的含量；免疫组化法检测心肌诱导型一氧化氮合酶(inducible nitric oxide synthase，iNOS)和原癌基因(cellular oncogene fos，c-fos)阳性蛋白表达。结果：SHR组心肌细胞增生肥大且排列紊乱，组织中可见大量炎性细胞和脂肪组织浸润，心肌T-SOD的含量明显减少(P<0.01)，心肌iNOS和c-fos蛋白表达显著增加(P<0.01)，芝麻素和卡托普利组上述病理变化则有不同程度的改善；心肌T-SOD明显增加(P<0.01)，iNOS和c-fos蛋白表达明显下调(P<0.01)。结论：芝麻素具有下调SHR心肌iNOS和c-fos蛋白表达，增强T-SOD能力，保护心肌损伤的作用。

【关键词】芝麻素；自发性高血压大鼠；心肌；T-SOD；iNOS；c-fos

【文献标识码】【中图号】R 285.5A

DOI【】10.3969/j.issn.1002-0217.2015.02.001

文章编号：1002-0217(2015)02-0107-03

基金项目：安徽省大学生创新创业训练计划项目(AH201310368112)

收稿日期：2014-07-16

作者简介：曹雨朦(1993-),女，2011级本科生，(电话)0553-3932464，(电子信箱)892466332@qq.com；

Effect of sesamin on the protein iNOS and c-fos expression in myocardia of spontaneouslyhypertensive ratsLIWei,YANGJieren,LIWenxing,ZHANGJunxiu,TANGLijuan,YANGHui,XIONGYin

Department of Pharmacology,Wannan Medical College,Wuhu 241002,China

Abstract【】Objective：To observe the effects of sesamin on the protein iNOS and c-fos expression in myocardia of spontaneously hypertensive rats(SHR),and explore the potential protective mechanisms.Methods:Thirty-five SHRs were randomly divided into group of SHR model,sesamin treatment in diverse dosage[40,80 and 160 mg/(kg·d)] and captopril intervention[30 mg/(kg·d)],and 7 Wistar-Kyoto rats(WKY) were included as negative controls.All rats were intragastrically administrated with the drugs once a day for consecutive 16 weeks.After the final intervention,the body weight (BW) was measured,and histopathological changes of the myocardia were observed by HE staining.Colorimetric analysis was performed to measure the contents of myocardial T-SOD,and immunohitochemical method was used to determine the expression of inducible nitric oxide synthase(iNOS) and c-fos.Results:SHR group demonstrated hypertrophied myocardia with disordered arrangement.Large quantity of inflammatory cells and adipose tissue infiltration were seen in the myocardial tissues,and the expression of T-SOD was significantly reduced (P<0.01),whereas the protein iNOS and c-fos expression in myocardia was significantly up-regulated(P<0.01).Myocardial pathological lesions were improved to a certain degree in the rats treated with sesamin and captopril,and T-SOD content was significantly increased (P<0.01),whereas the expression of iNOS and c-fos was notably reduced in dose-dependent manner(P<0.01).Conclusion:Sesamin could decrease the expression of iNOS and c-fos protein and enhance the ability of T-SOD,suggesting that it can be protective effect on myocardial injury in spontaneously hypertensive rats.

【Key words】sesamin;spontaneously hypertensive rats;myocardium;T-SOD;iNOS;c-fos

高血压病是一种危害人类健康的常见病，长期高血压可导致重要靶器官损伤，尤其对心脏损伤更为严重，可引起心肌细胞肥大、间质纤维化、细胞外基质增加、心肌细胞凋亡、心脏扩大，最终导致心脏衰竭。故临床上对高血压的治疗不仅需要降压，同时要考虑保护靶器官。芝麻素(sesamin，Ses)是从芝麻中提取的活性有效成分，具有抗氧化、降血脂、降血压等[1-3]作用。国外报道芝麻素不仅可以降低血压，还能改善去氧皮质酮-盐敏感性高血压及两肾一夹肾性高血压大鼠的心肌和血管的肥厚[4-5]，我们前期研究表明，芝麻素可以逆转肾性高血压、肾性高血压伴高脂高糖饮食大鼠及SHR心肌肥厚的作用[6-8]。……