滨蒿总黄酮抗炎、镇咳、解热作用的实验研究

史银基1,2， 司丽君2, 刘燕2， 杨巧丽2， 姚华2， 史玉柱2， 王雪2， 顾政一2， 黄华2

(1新疆医科大学研究生院， 乌鲁木齐830011；2新疆维吾尔自治区药物研究所， 乌鲁木齐830004)

摘要：目的研究滨蒿总黄酮的抗炎、镇咳、解热作用。方法通过二甲苯致小鼠耳廓肿胀模型和冰醋酸致小鼠腹腔毛细血管炎性渗出模型，观察滨蒿总黄酮的抗炎作用；采用氨水引咳法和枸橼酸引咳法，观察其镇咳作用；通过干酵母诱导的大鼠发热模型，考察其解热作用。结果滨蒿总黄酮对小鼠耳廓炎性肿胀有明显的抑制作用，0.2、0.4 g/kg滨蒿总黄酮的肿胀抑制率均为29.4%；对冰醋酸致小鼠腹腔毛细血管炎性渗出也有一定的抑制作用，0.4 g/kg滨蒿总黄酮的抑制率为29.9%；滨蒿总黄酮对氨水致小鼠咳嗽反应有一定的镇咳作用，0.2、0.4 g/kg滨蒿总黄酮对咳嗽反应的抑制率为22.2%、35.6%；滨蒿总黄酮对枸橼酸引起的豚鼠咳嗽反应有较强的镇咳作用，0.1、0.2、0.4 g/kg滨蒿总黄酮对咳嗽反应的抑制率分别为32.1%、41.3%、49.0%；滨蒿总黄酮还可缓解酵母引起的大鼠发热反应，0.2、0.4 g/kg滨蒿总黄酮在大鼠致热后5.5～7 h(即给药后2.5～4 h)时间段有明显的降温作用。结论滨蒿总黄酮具有良好的抗炎、镇咳及解热作用。

关键词：滨蒿总黄酮； 抗炎； 镇咳； 解热

中图分类号：R965.1文献标识码：A

doi:10.3969/j.issn.1009-5551.2015.05.013

[收稿日期:2015-03-10]

基金项目：乌鲁木齐市科技攻关计划(G121120002)

作者简介：杨丽萍(1989-)，女，硕士，研究方向：新药研发。

Effects of total flavonoids from Artemisia Scoparia on anti-inflammatory,

relieving cough and antipyretic

SHI Yinji1,2， SI Lijun2， GU Zhengyi2， LIU Yan2， YANG Qiaoli2， YAO Hua2，

WANG Xue2， SHI Yuzhu2， HUANG Hua2

(1XinjiangMedicalUniversity,Urumqi830011,China;2XinjiangInstituteofMateriaMedic,

Urumqi830004,China)

Abstract:ObjectiveTo observe the effects of total flavonoids from Artemisia Scoparia on anti-inflammatory, relieving cough and antipyretic. MethodsThrough the tests of ear conch induced by xylene and glacial acetic acid-induced mouse peritoneal capillary inflammatory exudate model, we observed the anti-inflammatory effects of total flavonoids from Artemisia Scoparia. Relieving cough caused by the irritation of ammonia water in mice and cough model induced by citric acid in guinea pigs were used to observe the antitussive action. The models of dry yeast-induced fevered rats was used to study the antipyretic effects. ResultsTotal flavonoids from Artemisia Scoparia could inhibit the degree of ear conch induced by xylene, dose of 0.2 g/kg, 0.4 g/kg could inhibit the mice auricle inflammatory swelling inhibition rate was 29.37%; Inhibit the degree of glacial acetic acid-induced mouse peritoneal capillary inflammatory exudate model also, dose of 0.4 g/kg inflammatory swelling inhibition rate was 29.9%; The mice cough response inhibition rate was 22.2% and 35.6%，with dose of 0.2 g/kg and 0.4 g/kg of total flavonoids from Artemisia Scoparia; Dose of 0.1 g/kg，0.2 g/kg，0.4 g/kg of total flavonoids from Artemisia Scoparia to guinea pig cough caused by citric acid inhibition rates were 32.1%, 41.3% and 49.0% respectively; Total flavonoids from Artemisia Scoparia have obvious antipyretic effect, dose of 0.2 g/kg，0.4g/kg of total flavonoids from Artemisia Scoparia had best cooling effect that rats fever model induced after 5.5h to 7h (I.e., 2.5 to 4 hours after administration). ConclusionThe total flavonoids from Artemisia Scoparia had better effect on anti-inflammatory, relieving cough and antipyretic.

Key words: the total flavonoids from Artemisia scoparia; anti-inflammatory; relieving cough; antipyretic

滨蒿(Artemisia scoparia Waldst.et kit)为菊科蒿属植物，维吾尔名“西瓦克”，是传统的维吾尔药材，也是中药茵陈的植物来源之一，在新疆分布极为广泛。……