蒙药额日敦乌日勒对大鼠周围神经损伤早期c-fos表达及神经功能的影响

额尔敦朝鲁，包春生，周铁宝，赵福全，胡玉荣，李立

(内蒙古民族大学附属医院，内蒙古 通辽 028007)

摘要：目的观察额日敦乌日勒对大鼠坐骨神经损伤早期c-fos表达及神经功能的影响，探讨其疗效及作用机理。方法将64只SD大鼠随机分为假手术组、模型组、阳性对照组、额日敦乌日勒组。除了假手术组外建立坐骨神经夹持损伤模型，阳性对照组大鼠每日给予维生素B1和维生素B12各3 mg/kg、10 μg/kg；额日敦乌日勒组大鼠每日给予额日敦乌日勒0．3 g/kg，灌胃体积均为2 mL/100 g，观察造模后1周、4周每组大鼠坐骨神经功能指数(SFI)、神经传导速度(NCV)及坐骨神经中c-fos表达变化。结果治疗1周后，假手术组、阳性对照组、额日敦乌日勒组SFI值较模型组差异有统计学意义(P<0．05),且额日敦乌日勒组优于阳性对照组 (P<0．05) ；额日敦乌日勒组、阳性对照组与模型组比较，NCV值有所上升(P<0．05)；额日敦乌日勒组、阳性对照组与模型组比较，c-fos蛋白表达灰度值均明显增高(P<0．05)。治疗4周后，模型组、假手术组、阳性对照组、额日敦乌日勒组SFI值差异无统计学意义(P> 0．01)，额日敦乌日勒组、阳性对照组与模型组比较，NCV值明显提高(P<0．05)；额日敦乌日勒组、阳性对照组与模型组比较,c-fos蛋白表达灰度值均明显增高(P<0．05)。结论额日敦乌日勒对周围神经损伤有一定的疗效，其作用机制可能是通过抑制c-fos表达,从而起到促进坐骨神经感觉和运动功能恢复的作用。

关键词：额日敦乌日勒；坐骨神经损伤；坐骨神经功能指数；免疫组化；c-fos蛋白

DOI:10.13463/j.cnki.jlzyy.2015.11.022

中图分类号：R722.14文献标志码： A

基金项目：内蒙古自治区卫生厅项目(2005061)。

作者简介：额尔敦朝鲁(1963-)，男,医学硕士，教授，主任医师，主要从事神经系统感染免疫性疾病研究。

收稿日期:(责任编辑：张瑞彬2014-11-14)

Erdunwurile on C-fos expression and nerve function of early peripheral nerve injury in rats

Eerdunchaolu, BAO Chunsheng, ZHOU Tiebao, ZHAO Fuquan, HU Yurong, LI Li

(Affiliated Hospital of Inner Mongolia University for Nationalities, Tongliao 028007,

Inner Mongolia Autonomous Region,China)

Abstract：ObjectiveThe effects of Eridunwurile on c-fos expression and the neural function of Early stage following sciatic nerve injury in rats are observed through experiments, to explore the effects and mechanism of it. MethodsThe 64 SD rats were randomly divided into sham-operated group,model group, positive control group and Eridunwurile group. In addition to sham-operated group was established sciatic nerve injury model,positive control group's rats was given 3 mg/kg,10 μg/kg of vitamin B1,and vitamin B12;Erdunwurile group’s rats was given 0.3 g/kg,2 mL/100 g.At 1st and 4th weeks after treatment, 8 rats from each group detected sciatic nerve function index(SFI), and nerve conduction velocity (NCV) and grey value of c-fos positive expression. ResultsSFI: At 1st week after treatment, the recovery of Eridunwurile low-dose group, Eridunwurile high-dose group and positive control group was obviously superior to the model group,there is statistically significant (P<0．05); Eridunwurile low-dose group was significantly different from positive control group (P<0．05). TWL: At 1st week after treatment, compared with model group, TWL of Eridunwurile low-dose group were significantly prolonged. (P<0．05). NCV: At 1th and 4th weeks after treatment, compared with the model group, the NCV of Eridunwurile low-dose group and positive control group were obviously accelerated(P<0．05). After 4 weeks treatment Model group, control group, positive control group, the forehead, SFI value, le group no significant statistically(P> 0．01), Grey value of C-FOS positive expression：At 1st and 4th weeks after treatment, Gray value of Eridunwurile low-dose group, Eridunwurile high-dose group and positive control group was significantly higher than the model group (P<0．05); Gray value of Eridunwurile low-dose group was significantly higher than the positive control group(P<0．05). ConclusionErdunwurle to be effective treat sciatic nerve injury in rats, its mechanism is improve the conductive function, analgesia and recovery of sciatic nerve functional by inhibited c-fos expression.

Keywords：Eridunwurile;sciatic nerve injury; thermal withdrawal latency;sciatic nerve cduction velocity;c-fos protein

周围神经损伤是因直接或间接因素(如外伤、感染、免疫、代谢障碍等)引起神经干或其分支损伤而导致神经功能缺失。周围神经损伤再修复是一个复杂的过程，研究表明c-fos 表达参与轴突对伤害性刺激的反应，对神经受到外界刺激后能够迅速作出反应而进行表达[1]。……