四逆汤对腹主动脉缩窄大鼠血压和压力反射敏感性的影响

吴 琼,刘永明,高俊杰,邵春林,沈智杰,陈铁军,郭 蔚,王英杰,王肖龙

摘要:目的 研究四逆汤方对腹主动脉缩窄大鼠血压和压力反射敏感性(BRS)的影响。方法 采用腹主动脉缩窄法制备高血压大鼠模型50只,造模成功后随机分为模型组(M组)、假手术组(B组)、四逆汤组(S组)、四逆汤+培哚普利组(SP组)、培哚普利组(P组)共5组,每组10只。术后4周开始灌胃,以假手术组为空白对照组,培哚普利组为阳性药物对照组,药物干预8周。用尾动脉仪连续动态测量大鼠左前肢血压。干预后8周通过股动脉插管,股静脉注射苯肾上腺素观察大鼠BRS、血压变化。结果 造模后4周,与假手术组相比,各组收缩压均显著增高(P<0.001)、舒张压增高不明显(P>0.05),其中四逆汤组、四逆汤+培哚普利组舒张压增高(P<0.05)。药物干预后4周,与模型组相比,四逆汤组、四逆汤+培哚普利组、培哚普利组收缩压均明显降低(P< 0.001),舒张压虽无明显降低,但与造模后血压相比有所下降(P<0.05)。药物干预后8周,与前一时间点血压相比,各组血压均无进一步降低(P>0.05)。药物干预后,与模型组相比,四逆汤组、四逆汤+培哚普利组、培哚普利组BRS明显上升(P<0.001)。结论 四逆汤可改善腹主动脉缩窄大鼠的血压,可增加腹主动脉缩窄大鼠压力反射敏感性。

关键词:腹主动脉缩窄;四逆汤;压力反射敏感性;血压

中图分类号:R544.1 R285.5

文献标识码:A

doi:10.3969/j.issn.1672-1349.2015.12.008

文章编号:1672-1349(2015)12-1381-04

基金项目:上海市中医临床重点实验室基金项目(No.14DZ2273200)

通讯作者:王肖龙,E mail:wxlqy0214@163.com

Abstract:Objective To study the effects of Sini decoction(SND)on blood pressure and baroreflex sensitivity(BRS)in rats with abdominal aortic coarctation.Methods Fifty hypertension rats were established by abdominal aortic coarctation operation.After mode-l ing,rats were randomly divided into 5 groups:Sham operation group,SND group,SND plus perindopril group,perindopril group and model group.Rats were gavaged 4 weeks later.The sham operation group was the control group,perindopril as the positive control group.Caudal artery with continuous dynamic measurement instrument left forelimb blood pressure in rats.By cannulating into femoral artery,injecting phenylephrine into femoral artery,we observe the change of BRS,MAP,and HR.Results After modeling of 4 weeks,compared with the control group,the systolic blood pressure were significantly increased in each group(P<0.001).After drug intervention of 4 weeks,compared with the model group,the systolic blood pressure were significantly lower in SND group,SND plus perindopril group,perindopril group(P<0.001).The diastolic blood pressure was lower after modeling(P<0.05).After drug intervention,compared with model group,the BRS obviously increased in SND group,SND plus perindopril group,perindopril group(P<0.001).Conclusion SND can improve the blood pressure and increase the baroreflex sensitivity in rats with abdominal aortic coarctation.

作者单位:上海中医药大学附属曙光医院(上海201203)

Influence of Sini Decoction on Blood Pressure and Baroreflex Sensitivity in Rats with Abdominal Aortic Coarctation

Wu Qiong,Liu Yongming,Gao Junjie,Shao Chunlin,Shen Zhijie,Chen Tiejun,Guo Wei,Wang Yingjie,Wang Xiaolong

Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China

Corresponding Author:Wang Xiaolong

Key words:abdominal aortic coarctation;Sini decoction;baroreflex sensitivity;blood pressure

压力反射敏感性(baroreflex sensitivity,BRS)是血压变化导致反射性心率变化的敏感程度,反映血压升高所引起的心脏迷走神经反射调节情况。大量研究证实BRS降低可能是血压变异性(BPV)升高的原因之一,同时也是导致原发性高血压发生发展的原因之一。本实验室前期研究发现四逆汤能够改善高血压患者的BPV,而BPV与BRS呈负相关。……