天然二倍体和四倍体泥鳅鳍细胞系染色体组构成研究

李雅娟1，隋燚1，赵睿2，李霞1，周贺1，刘博1，马海艳1

（1.大连海洋大学辽宁省海洋生物资源恢复及生境修复重点实验室，辽宁大连116023；2.北京市水产技术推广站，北京101105）

摘要：对传代20次的天然二倍体和天然四倍体泥鳅鳍细胞系进行染色体标本制备，并对其染色体核型、Ag-NORs及CMA3/DA/DAPI三重荧光染色等进行研究。结果显示，①二倍体泥鳅鳍组织培养细胞的染色体数目为2n=50，核型公式为：2n=10m+4sm+36t，NF=64；四倍体泥鳅鳍组织培养细胞的染色体数目为4n=100，核型公式为：4n=20m+8sm+72t，NF=128。②天然二倍体泥鳅鳍培养的细胞中期染色体分裂相有2个Ag-NORs，天然四倍体泥鳅鳍培养的细胞中期染色体分裂相有4个Ag-NORs，均位于第1对中部着丝粒染色体（M1）的末端。③天然二倍体泥鳅鳍培养的细胞染色体中期分裂相中CMA3阳性位点为2个，天然四倍体泥鳅鳍培养的细胞染色体中期分裂相中CMA3阳性位点为4个；均位于核仁组织区，即第1对中部着丝粒染色体（M1）的末端。结果表明，天然二倍体和四倍体泥鳅鳍培养细胞制备的染色体数目、核型和带型与体细胞一致。

关键词：泥鳅；二倍体；四倍体；细胞系；染色体组；Ag-NORs；CMA3/DA/DAPI

网络出版时间2015-4-23 12:15:18

[URL]http://www.cnki.net/kcms/detail/23.1391.S.20150423.1215.011.html

李雅娟,隋燚,赵睿,等.天然二倍体和四倍体泥鳅鳍细胞系染色体组构成研究[J].东北农业大学学报, 2015, 46(4): 83-88.

Li Yajuan, Sui Yi, Zhao Rui, et al. Study on genosome constitution of natural diploid and tetraploid loach Misgurnus anguillicaudatus fin cells line[J]. Journal of Northeast Agricultural University, 2015, 46(4): 83-88. (in Chinese with English abstract)

Study on genosome constitution of natural diploid and tetraploid loach

Misgurnus anguillicaudatus fin cells line

/LI Yajuan1, SUI Yi1, ZHAO Rui2, LI Xia1, ZHOU He1, LIU Bo1, MA Haiyan

1

(1. Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Dalian Liaoning 116023, China; 2. Fisheries Technology Extension Station of Beijing, Beijing 101105, China)

Abstract:Fin cells of natural diploid and tetraploid loaches subcultured after 20 generations were used for chromosome preparation, and karyotype, Ag-NORs, and CMA3/DA/DAPI were studied primarily. Results showed as follows:①Chromosome number of diploid cells was 2n=50, karyotype was 2n=10m+4sm+ 36t, NF=64; and chromosome number of tetraploid was 4n=100, karyotype was 4n=20m+8sm+72t. NF= 128.②There were 2 Ag-NORs in the medium chromosome spread of diploid, and 4 in tetraploid, all in the terminal of the first metacentric chromosome(M1).③Positive CMA3sites in diploid were 2, and 4 in tetraploid, all in the neuclear organizer region, as the terminal of the first metacentric chromosome(M1). In conclusion, chromosome number, karyotype and band type of cultured cells of both diploid and tetraploid were consistent with somatic cells. Therefore, methods in this study could be of great value for researches of cytogenetic and molecular genetics of loaches.

Key words: Misgurnus anguillicaudatus; diploid; tetraploid; cell line; genome; Ag-NORs; CMA3/DA/DAPI

泥鳅（Misgurnus anguillicaudatus）是我国常见的小型经济鱼类，具有肉质细嫩，味道鲜美、营养丰富、易繁殖、抗性强等特点，是我国主要养殖鱼类品种之一。

鱼类细胞培养技术广泛应用于环境毒理学、病毒学、肿瘤学、细胞生物学、资源保护以及遗传学等领域。……