林锦锋　杨志业　李曼莎等

[摘要] 目的 研制薄层色谱鉴别用巴戟天对照提取物，以解决对照药材的局限性。 方法 从原料选定、制备工艺研究、稳定性影响因素考察等方面探讨薄层色谱鉴别用巴戟天对照提取物的研究思路，并比较巴戟天对照药材与对照提取物在薄层鉴别中的应用结果。 结果 巴戟天薄层鉴别用对照提取物的提取条件：巴戟天粗粉加20倍70%乙醇，加热回流提取，提取2次，每次1 h。该对照提取物替代对照药材应用于巴戟天及其成方制剂中的薄层鉴别效果一致。 结论 本研究制备的巴戟天薄层鉴别用对照提取物，可以替代巴戟天对照药材用于巴戟天及其成方制剂的质量控制分析。

[关键词] 巴戟天；薄层色谱鉴别；对照提取物；质量控制

[中图分类号] R927.11 [文献标识码] A [文章编号] 1673-7210（2015）09（c）-0022-05

[Abstract] Objective To develop Morinda officinalis How reference extract for TLC to solve the limitation of reference crude herb. Methods The research strategy of Morinda officinalis How reference extract for TLC was discussed from raw material selected， preparation technology and stability influence factors. Practical results between Morinda officinalis How reference crude drug and reference extract in TLC were compared. Results The extract condition of reference extract used for TLC of Morinda officinalis How was as follows： Morindae officinalis How thick powder was heating reflux extracted with 20 flods of 70% ethanol for two times， 1 hour each time. The reference extract instead of reference crude drug used for TLC of Morindae officinalis How and its historical preparations gained the same effect. Conclusion The reference extract of Morindae officinalis How for TLC prepared by this study can replace Morindae officinalis How reference crude herb to be used for quality control analysis of Morindae officinalis How and its historical preparation.

[Key words] Morindae officinalis How； TLC identification； Reference extract； Quality control

巴戟天为茜草科植物巴戟天Morinda officinalis How的干燥根，始载于《神农本草经》，列为上品，为较常用的中药，有补肾壮阳、强筋骨、祛风湿的作用，可用于阳痿遗精、宫冷不孕、月经不调、小腹冷痛、风湿痹痛、筋骨痿软[1-5]。其以肉质根入药，是我国著名的四大南药之一，在中医药处方中应用广泛，在我国南方地区以及港澳台地区还作为常用的食疗补品，为我国主要出口药材之一[6]。

目前，在《中国药典》及国家、地方的其他药材和成药标准中，主要采用对照药材和中药单体成分对照品作为薄层色谱鉴别项的对照物质，这种模式存在如下局限性：①对照药材品种有的黏度大难以粉碎均匀，粉碎后的稳定性降低，使用量过大，前处理麻烦或提取得到的供试品溶液含多种成分黏稠而难以点样等；……