乔婵 万秀清 李若 魏亭宇 吴国顺 都兴洋 黄磊

摘要

[目的]筛选与鉴定烟草抗PVY病突变体，为寻找抗PVY的基因奠定基础。[方法]通过苗期对烟草突变材料进行PVY接种鉴定，肉眼对材料进行初步筛选，对筛选到的抗性材料进一步荧光定量鉴定，对筛选鉴定的抗性材料进行苗期鉴定及田间抗性鉴定。[结果]2011年筛选获得2个高抗PVY的突变体MZE215和MZE216，以及3个耐PVY突变体MZE270、MZE2207及MZE2228;2012年进一步筛选鉴定，突变体材料MZE2407和MZE2428感PVY，MZE216和2份MZE215突变体材料抗PVY。[结论]试验结果为控制烟草PVY病的危害提供了理论依据。

关键词 烟草;PVY;突变体

中图分类号 S572  文献标识码 A  文章编号 0517-6611（2015）03-099-03

Selection and Identification of Tobacco Mutants with PVY Resistance

QIAO Chan， WAN Xiuqing*， LI Ruo et al

（Mudanjiang Tobacco Research Institute，Mudanjiang， Heilongjiang 157011）

Abstract [Objective] Tobacco mutants with PVY resistance were selected and identified to lay the basis for finding PVY resistant genes. [Method] Different tobacco mutants were inoculated with PVY in seedling stage and were preliminarily selected by nakedeye observation. Enzymelinked immunosorbent assay （ELISA）， realtime fluorescence quantitative PCR and test strip assay were performed to further identify preselected mutants. Finally， resistance of these identified mutants was evaluated by pot culturing in green house and in the open field. [Result] Two mutants MZE215 and MZE216 showing high PVY resistance and three mutants MZE270， MZE2207and MZE2228 with tolerant PVY resistance were obtained in 2011. These mutants were further identified in 2012， and results showed that MZE2407 and MZE2428 were PVY susceptible， whereas MZE216 and MZE215 were PVY resistant. [Conclusion] The results provide theoretical basis for the control of tobacco PVY.

Key words  Tobacco; PVY; Mutants

基金项目 国家局烟草基因组计划重大专项突变体项目[110201201004（JY04）]。

作者簡介

乔婵（1978-），女，黑龙江双城人，农艺师，硕士，从事烟草分子生物学和病理学研究。*通讯作者，高级农艺师，硕士，从事烟草分子生物学和病理学研究。

收稿日期 20141203

烟草PVY病又称为叶脉坏死病、烟草脉帯病、黄斑病，是能系统侵染烟草的病害^[1-2]。烟草PVY病在我国分布广，危害重，对烟草生产的危害正逐年加重，近年来更是在多个烟区呈急剧上升趋势，已成为危害我国各烟区烟草的主要病毒病害之一^[3-4]。烟草一旦感染PVY，整片叶子几乎失去烘烤价值，严重情况下烟茎坏死、叶片坏死，每公顷产值减少11%～18%;除影响产量外，被PVY侵染后的烟叶全氮含量增加，烟碱含量升高，尼古丁含量增加，糖氮比降低，还原糖减少，烤晒后的色泽及气味等品质均大大降低，烟叶等级指数也降低36%～62%^[5-6];PVY侵染烟草引起的PVY病严重影响烟叶的产量和质量，并造成较大的经济损失，严重影响烤烟生产的可持续发展^[7]。……