方波等

[摘要] 目的 寻找一种新的方法对同种异体肌腱进行进一步的预处理来改善其修复效果，为临床应用提供实验基础和理论依据。 方法 采用超深低温冷冻法获得兔同种异体肌腱，应用碳化二亚胺对其进行交联并肝素化，检测其组织相容性；将交联组和未交联组肌腱对兔跟腱损伤进行修复，观察并对比不同时间段两组的腱骨愈合情况。结果1、4周EDC交联组炎症反应分级（Ⅰ～Ⅱ）均较未交联组（Ⅲ～Ⅳ）轻，差异有统计学意义（P值分别为0.020、0.030）；移植实验可见交联并肝素化的肌腱在修复跟腱损伤中的愈合能力比未交联的肌腱强，Sharpeys纤维出现早，缩短了腱骨愈合时间。 结论 经超深低温冷冻和EDC交联共同处理肌腱，增强其稳定性，降低了同种异体肌腱的免疫原性，促进肌腱与骨的愈合。

[关键词] 同种异体肌腱；超深低温冷冻；碳化二亚胺；移植

[中图分类号] R686.1 [文献标识码] A [文章编号] 1674-0742（2015）03（a）-0016-04

Empirical Study of Rabbit's Tendon Allograft Obtained by Ultra-deep Frozen and Cross-linked by EDC/NHS Repairing Injure of Achilles Tendon

FANG Bo CI Yuan ZONG Gang

Second People's Hospital of Dalian City， Dalian， Liaoning Province， 116000， China

[Abstract] Objective To find a new way to improve the repairing effect by further pretreating tendon allograft so as to provide an experimental basis and theory evidence for clinical application. Methods The rabbit's tendon allograft was obtained by ultra-deep frozen then cross-linked and heparinized by EDC/NHS and detected histocompatibility. Injure of rabbit achilles tendon was repaired by the tendon allograft of management and nulli-management. The aim was to observe and contrast tendon-bone healing of two sets in the different time. Results EDC crosslinking group grade inflammation（Ⅰ～Ⅱ） was lighter than those of uncross-linked group（Ⅲ～Ⅳ） at 1 or 4 weeks，the difference was statistically significant （P values were 0.020， 0.030）； the transplantation showed that the capability of agglutination of the tendon allograft of management was more powerful than nulli-management set on repairing injure. The time of Sharpey's fibers appearing was earlier， and the cross-linking and heparinization process can shorten tendon-bone healing time. Conclusion Tendon allograft obtained by ultra-deep frozen and cross-linked by EDC/NHS reinforces its constancy and reduces immunogenicity as well as promotes tendon-bone healing.

[Key words] Tendon allograft； Ultra-deep frozen； Carbodiimide； Transplant

目前运动损伤、交通意外造成的跟腱损伤明显增多，处理这类损伤目前主要采用自体肌腱移植重建。由于自体肌腱取材时会造成一定程度的副损伤，且自体组织来源有限，使得同种异体肌腱的研究得以快速发展。而同种异体肌腱移植成功的关键是如何降低或抑制移植物的抗原性。该实验通过超深低温冷冻及应用碳化二亚胺处理肌腱以探求其降低同种异体肌腱抗原性的可能性，为进一步开展临床研究奠定基础，报道如下。

1 实验方法

1.1 同种异体肌腱的制备

实验于2011—2013年之间进……