林晗婧，田　野，张秀丽，孙广玉（东北林业大学生命科学学院，哈尔滨150040）

弱光环境对罗勒幼苗叶片生长和叶绿素荧光特性的影响

林晗婧，田野，张秀丽，孙广玉
（东北林业大学生命科学学院，哈尔滨150040）

以花色素苷含量不同的紫罗勒和大叶罗勒为对象，分别对其进行弱光和自然光处理30d后进行生长参数、色素含量和叶绿素荧光参数的测定，结果显示：弱光导致两种罗勒幼苗叶片比叶面积、叶面积、叶宽、叶长和株高显著增加，幼苗根长，地上、地下生物量和总生物量明显降低。经过弱光处理的叶片花色苷与总叶绿素比和花色苷含量均降低。总叶绿素含量、叶绿素a含量、叶绿素b含量和叶绿素a/b比值较自然光明显升高。在弱光下，两种罗勒幼苗叶片失活PSⅡ反应中心的热耗散量子产额（ФNF）降低，而基本的荧光量子产额和热耗散的量子产额（Фf，D）升高。说明，花色素苷可能在光破坏防御上发挥了作用。

罗勒；弱光；叶绿素荧光；花色素苷

Abstract∶Based onOcimum basilicumL.seedlings with different content of anthocyanins，this article studied on the growth parameters，pigment content and chlorophyll fluorescence parameters dealed with lowlight intensity and nature light intensity for 30 days.Finally，the specific leaf area，leaf area，blade width，leaf length，plant height of leaves ofOcimum basilicumL.seedlings increased significantly，however root length，aboveground biomass，underground biomass，total biomass of leaves decreased significantly with low light intensity.The content of anthocyanins and the ratio of anthocyanins and total chlorophyll ofOcimum basilicumL.seedlings with low light intensity was lower than the one with nature light intensity，however the content of total chlorophyll，chlorophyll a，chlorophyll b，the ratio ofchlorophyll a and b ofOcimum basilicumL.seedlings with lowlight intensity was higher. Dealed with lowlight intensity，the quantum yield of thermal dissipation in nonfunctional PSⅡ（ФNF）decreased，the basic quantum yield of fluorescence and thermal dissipation（Фf，D）increased in leaves ofOcimum basilicumL. seedlings.The result showed that anthocyanins mayhave the effect on the defense oflight damage.

Key words∶OcimumbasilicumL；lowlight density；chlorophyll fluorescence；anthocyanin

罗勒（Ocimum basilicum L.）为唇形科罗勒属一年生草本植物，原产于南洋群岛及非洲等地，遍布全球［1］。罗勒形态上呈扇形植株，枝繁叶茂，紫花成串，适宜盆栽，由于其具有能香化、净化空气、驱蚊等功效，而被誉为“天然香水瓶”［2］。由于罗勒是一种值得推广的天然保健、药食同源的资源植物，因而近年来市场需求不断增大，食用罗勒栽培面积亦呈增长趋势［3］。紫罗勒是罗勒的变种之一，其叶片富含大量的花色素苷，该类物质属于天然色素类抗氧化剂，可被应用于食品以及药品等领域［4］，其主要分布于幼嫩叶片和衰老叶片中。……