李铁浪，粟艳梅，祁芳，倪伟，张泓*，张雨辰，郭华（.湖南中医药大学针灸推拿学院，湖南长沙4008；.娄底市中心医院康复科，湖南娄底47000；.湖南省中医院疼痛理疗科，湖南长沙40005）

艾灸对脾虚胃溃疡模型大鼠血清TFF及胃黏膜ERK1/2、PCNA的影响

李铁浪1，粟艳梅2，祁芳1，倪伟1，张泓1*，张雨辰3，郭华1
（1.湖南中医药大学针灸推拿学院，湖南长沙410208；2.娄底市中心医院康复科，湖南娄底417000；3.湖南省中医院疼痛理疗科，湖南长沙410005）

目的观察艾灸对脾虚胃溃疡模型大鼠血清三叶因子（TFF1、TFF2）、胃黏膜细胞外信号调节激酶1/2（ERK1/2）和增殖细胞抗原（PCNA）的影响，探讨艾灸促进胃黏膜增殖修复的可能机制。方法将50只健康SD大鼠随机抽取10只为空白组，其余40只采用苦寒泻下法+无水乙醇灌胃建立脾虚胃溃疡大鼠模型，模型成立后随机分为模型组、四君子汤组、艾灸组和艾灸非穴位组，每组10只。各组予以相应处理，8 d后采用酶联免疫吸附法检测血清中TFF1、TFF2的含量，免疫印迹法检测胃黏膜组织p-ERK1/2蛋白的表达，免疫组化法测胃黏膜细胞PCNA的表达。结果艾灸足三里、中脘等穴能明显提高胃黏膜损伤大鼠血清TFF1、TFF2的含量，增强胃黏膜损伤大鼠胃黏膜组织p-ERK1/2蛋白和PCNA的表达（P＜0.05或P＜0.01）。结论艾灸能促进脾虚胃溃疡大鼠损伤胃黏膜的增殖修复，其可能的机制是通过提高TFF1、TFF2的含量，激活TFF/ERK信号转导通路，增强PCNA的表达有关。

艾灸；胃黏膜增殖修复；三叶因子；细胞外信号调节激酶1/2；增殖细胞抗原

〔Abstract〕ObjectiveToobservetheeffectsofmoxibustiononcontentsofserumtrefoilfactor（TFF1，TFF2），andgastricmucosaextracellularsignal-regulatedkinase1/2（ERK1/2）andproliferatingcellnuclearantigen（PCNA）in spleendeficiencygastriculcerratmodels.Toexplorethepossiblemechanismofmoxibustioninpromotingmucosal proliferation repair.Methods 10 rats were randomly selected from the 50 healthy SD rats，the other 40 rats were built into the spleen deficiency gastric ulcer models by the cold diarrhea down+anhydrous ethanol gavage methods.After modeling，the ratswererandomlydividedintothemodelgroup，Sijunzidecoctiongroup，moxibustiongroupandnonacupoint-moxibustion group，10 in each group.The serum contents of TFF1 and TFF2 were detected by ELISA，the expression of gastric mucosa p-ERK1/2 protein was measured with Western blot，and the expression of gastric mucosa PCNA was determined by immunohistochemical method.Results Moxibustion Zusanli and Zhongwan points can significantly improve contents of TFF1 and TFF2 in rats with injury gastric mucosa，increase the expression of gastric mucosa p-ERK1/2 protein and PCNA in rats with injury gastric mucosal（P＜0.05 or P＜0.01）.Conclusion Moxibustion can promote the proliferation and repair of injury gastric mucosal in spleen deficiency gastric ulcer rats.The mechanism may be related with increasing the contents of TFF1 and TFF2，activating TFF/ERK signal transduction pathway，and enhancing the expression of PCNA.

〔Keywords〕moxibustio；mucosalproliferationrepair；trefoilfactor；extracellularsignal-regulatedkinase1/2；cell proliferation antigen

胃黏膜损伤后增殖修复是由多因素介导，并相互影响而形成的一个复杂的网络体系。……