张晓岚

[摘要] 目的 探讨RAD51及CHK1在喉鳞状细胞癌组织中的表达及意义。 方法 收集2012年1～12月于河北省眼科医院，经病理确诊且未经放化疗的喉鳞状细胞癌患者50例的癌组织、同一患者相对应的50例癌旁黏膜组织及17例非喉部恶性肿瘤疾病患者的喉部正常黏膜组织，采用免疫组化二步法检测标本组织中RAD51、CHK1的表达，分析二者间的关系。 结果 喉鳞状细胞癌组织中RAD51的阳性率（74.00%）明显高于相对应的癌旁黏膜组织（22.00%），差异有统计学意义（χ2=11.235，P = 0.014）；也高于非喉部恶性肿瘤疾病患者的喉部正常黏膜组织（17.64%），差异有高度统计学意义（χ2=12.346，P = 0.001）。喉鳞状细胞癌组织中CHK1阳性率（78.00%）高于癌旁黏膜组织（16.00%），差异有统计学意义（χ2=9.235，P = 0.041）；也高于正常黏膜组织（11.76%），差异有统计学意义（χ2=11.386，P = 0.010）；癌旁组织中CHK1的阳性率高于正常组织，但差异无统计学意义（χ2=1.245，P = 0.612）。RAD51的阳性高表达与喉鳞状细胞癌的病理分级、淋巴结的转移、临床分期有关（P < 0.05）；CHK1的阳性表达与喉鳞状细胞癌的病理分级、淋巴结的转移、临床分期有关（P < 0.05）。喉鳞状细胞癌组织中RAD51的表达与CHK1的表达呈正相关（r = 0.432，P < 0.01）。 结论 RAD51、CHK1在喉鳞状细胞癌组织中呈高表达，其表达与病理分级淋巴结的转移及临床分期有关，提示RAD51及CHK1的表达可能与喉鳞状细胞癌的预后和转移有关。

[关键词] 喉鳞状细胞癌；RAD51、CHK1；免疫组织化学

[中图分类号] R739.65 [文献标识码] A [文章编号] 1673-7210（2014）10（a）-0019-05

Epression and significance of RAD51 and CHK1 in laryngeal squamous cell carcinoma tissue

ZHANG Xiaolan

Department of Ear-nose-throat， Eye Hospital of Hebei Province， Hebei Province， Xingtai 054001， China

[Abstract] Objective To investigate the expression and significance of RAD51 and CHK1 in laryngeal squamous cell carcinoma tissue. Methods From January to December 2012， in Eye Hospital of Hebei Province， 50 cases of carcinoma tissue from 50 patiens with pathologically confirmed laryngeal squamous cell carcinoma without chemotherapy， 50 cases of cancer adjacent normal mucosa tissues form the same patients and 17 cases non-malignant diseases's normal mucosa tisssue were selected， the expression of RAD51 and CHK1 in these tissue were deteced by two-step immunohistochemical methods. The correlation between RAD51 and CHK1 were analyzed. Results The RAD51 positive rate of laryngeal squamous cell carcinoma tissue （74.00%） was significantly higher than that of the cancer adjacent normal mucosa tissue （22.00%）， the difference was statistically significant （χ2=11.235， P = 0.014）， also higher than that of the non-malignant diseases's normal mucosa tisssue （17.64%）， the difference was statistically significant （χ2=12.346，P = 0.001）. The CHK1 positive rate of laryngeal squamous cell carcinoma tissue （78.00%） was higher than that of the cancer adjacent normal mucosa tissue （16.00%）， the difference was statistically significant （χ2=9.235，P = 0.041）； aslo higher than that of the non-malignant diseases's normal mucosa tisssue （11.76%）， the difference was statistically significant （χ2=11.386，P = 0.010）； the CHK1 positive rate of the cancer adjacent normal mucosa tissue was higher than that of normal mucosa tisssue， but the difference was not statistically significant （χ2=1.245，P = 0.612）. The RAD51 expression positive rate of laryngeal squamous cell carcinom tissue had related with the pathological grade， lymph node metastasis and clinical stage （P < 0.05）. The CHK1 expression positive rate of laryngeal squamous cell carcinoma had related with pathological grade of laryngeal， lymph node metastasis and clinical stage （P < 0.05）. The expression of RAD51 of laryngeal squamous cell carcinoma tissue was positively correlated with CHK1 （r = 0.432， P < 0.01）. Conclusion RAD51 and CHK1 showes high expression in laryngeal squamous cell carcinoma tissue， and its expression closely related with histological grade， lymph node metastasis and TNM stage. It suggests that RAD51 and CHK1 expression may be associated with laryngeal cancer prognosis and metastasis.

[Key words] Laryngeal squamous cell carcinoma； RAD51； CHK1； Immunohistochemistry

喉鳞状细胞癌是头颈部肿瘤中常见的恶性肿瘤，恶性度高，易发生局部复发及远处转移，其发生、发展、预后及转移的研究已经成为了耳鼻咽喉-头颈外科的研究热点。细胞周期检测点激酶1（checkpoint kinase 1，CHK1）是在生物进化过程中极为保守的蛋白激酶，研究表明CHK1在人类许多恶性肿瘤中均有表达，可能与肿瘤的发生、发展及预后有关[1]。RAD51基因是细胞DNA修复系统中的重要组成部分，研究表明RAD51在人类许多恶性肿瘤中也呈高表达[2]。本研究应用免疫组织化……