陈日寿+丁秀芬

[摘要] 目的 本研究分析了莪术、鱼腥草、土茯苓三种中药对解脲支原体（Uu）临床分离株的体外抑菌作用，并确定了它们的最小抑菌浓度（MIC）。方法 采集临床标本，在体外培养获得Uu临床分离菌株，将三种中药各制成药液，用微量稀释法测定各中药对Uu临床分离菌株的最小抑菌浓度。 结果 鱼腥草MIC为3.90mg/mL，莪术MIC为3.90mg/mL，土茯苓MIC为7.81mg/mL。 结论 三种中药对Uu临床分离菌株均显示较好的体外抑制作用，效果以鱼腥草最佳，莪术次之，土茯苓最后。

[关键词] 中药；解脲支原体；最小抑菌浓度；体外作用

[中图分类号] R285.5   [文献标识码] A   [文章编号] 2095-0616（2014）11-36-03

An experimental study of the effects of three types of traditional Chinese medicine on in vitro inhibition of ureaplasma urealyticum

CHEN Rishou  DING Xiufen

Clinical Laboratory,Dongguan Hospital of Traditional Chinese Medicine,Dongguan 523000,China

[Abstract] Objective To analyze the in vitro antibacterial effect of three types of traditional Chinese medicine (TCM) of curcuma zedoary,houttuynia cordata and rhizoma smilacis glabrae on clinical isolates of ureaplasma urealyticum (Uu),and to confirm their minimum inhibitory concentration (MIC). MethodsClinical samples were collected and cultured in vitro to obtain clinical isolates of Uu.Three types of TCM were made into solutions,and the MIC of each TCM against clinical isolates were measured via microdilution method. Results The MIC of houttuynia cordata was 3.90 mg/mL,the MIC of curcuma zedoary was 3.90 mg/mL and the MIC of rhizoma smilacis glabrae was 7.81 mg/mL. Conclusion The three types of TCM shows a favorable in vitro inhibitory effect against clinical isolates of Uu.The houttuynia cordata has the best effect,the curcuma zedoary the second and the rhizoma smilacis glabrae,the worst.

[Key words] Traditional Chinese medicine(TCM);Ureaplasma urealyticum (Uu);Minimum inhibitory concentration (MIC);In vitro

解脲支原体（urea plasma urealyticum，Uu）是介于细菌和病毒之间、目前所知的能独立生活的最小微生物。它主要通过性接触传播，可导致非淋菌性尿道炎、子宫内膜炎等疾病，严重者可致女性不孕不育症、自然流产，且高浓度Uu感染的女性患不孕症的危险比正常人群相对高出近3倍[1]。临床上常用大环内酯类及喹诺酮类抗菌药治疗Uu感染，但由于广谱抗生素的广泛应用以及不正确使用等原因，Uu的耐药现象越来越常见。近年来以单味中药或复方中药制剂治疗泌尿生殖道Uu获得较为满意的疗效，且具有副作用少的优点[2-3]。据资料记载，莪术含有姜黄素、莪术醇、异莪术醇等20多种成分，在抗病毒、抗菌和抗肿瘤方面具有较好的作用[4-5]；鱼腥草化学成分复杂，主要成分有挥发油、生物碱、黄酮、多糖和有机酸等，具有较好的抗炎活性[6-8]；土茯苓含多种化学成分，有糖类、有机酸黄酮苷类、皂苷类及挥发油成分，具解毒利湿、抗菌消炎功效[9-11]。……