苏标+徐庆微+黄志力+张国祥

[摘要] 目的 探讨丁酸钠对乳腺癌细胞MCF-7生长的抑制作用。 方法 将乳腺癌细胞MCF-7常规培养至对数生长期，采用浓度为0、2.5、5.0、10.0mmol/L的丁酸钠处理后，观察对MCF生长的抑制作用。结果 浓度为2.5、5.0、10.0mmol/L的丁酸钠处理的MCF-7细胞生长速度明显慢于亲本细胞，随着浓度的增加呈减慢趋势，差异有统计学意义（P＜0.05）。浓度为5mmol/L的丁酸钠处理后的MCF-7细胞克隆形成率明显低于MCF亲本细胞，差异有统计学意义（P＜0.05）。丁酸钠处理的细胞G1明显高于亲本细胞，S期及G2/M期明显低于亲本细胞，凋亡细胞百分比明显高于亲本细胞，差异有统计学意义（P＜0.05）。 结论 丁酸钠可能是通过诱导MCF-7细胞凋亡而起到逆转其恶性生物学行为的作用。

[关键词] 丁酸钠；乳腺癌细胞；MCF-7；抑制

[中图分类号] R737.9   [文献标识码] A   [文章编号] 2095-0616（2014）11-33-04

The inhibitory effect of sodium butyrate on MCF-7 breast cancer cells growth

SU Biao  XU Qingwei  HUANG Zhili  ZHANG Guoxiang

Department of General Surgery, Guangzhou City Liwan District People's Hospital, Guangzhou 510000, China

[Abstract] Objective To investigate the inhibitory effect of sodium butyrate on the growth of breast cancer cells MCF-7. Methods Breast cancer MCF-7 cells were cultured to logarithmic growth phase, they were processed with sodium butyrate, 0, 2.5, 5.0, 10.0mmol/L were the concentration, the growth inhibition of MCF was observed. Results The growth rate of MCF-7 cell processed with the sodium butyrate with the Concentration 2.5, 5.0, 10.0mmol/L was significantly slower than that of the parent cell, the growth rate with increasing concentration was slower, there was the significant difference(P＜0.05). The colony efficiency of MCF-7 cells processed with the sodium butyrate with the concentration 5mmol/L was significantly lower than that of the parental cells, there was the significant difference (P＜0.05). After processed with sodium butyrate, Cell G1 was significantly higher than the parental cells, S phase and G2 / M phase were significantly lower than the parental cells, the percentage of apoptotic cells was significantly higher than the parental cells, there was the significant difference(P＜0.05). Conclusion Sodium butyrate may be by inducing apoptosis in MCF-7 cells and play a reversal of its role in the m alignant behavior.

[Key words] Butyrate; Breast cancer cells; MCF-7; Suppression

乳腺癌是一种常见的恶性肿瘤，严重影响女性的健康，近年来在世界范围内乳腺癌的发病率呈逐年上升趋势并且年龄逐渐年轻化[1-2]。丁酸钠为短链脂肪酸丁酸的钠盐，主要通过改变组蛋白的乙酰化程度来改变染色质结构，参与多种基因的表达[3]。部分食物在人体没有经过小肠消化，厌氧菌对上述食物中的碳水化合物及蛋白分解发酵后即可产生丁酸钠，其在结肠上皮细胞的能量来源中占有较大比例，使结肠肽或生长因子的释放受到刺激，对结肠黏膜血供进行调节，促进上皮细胞的增殖[4]，但有报道表明，丁酸钠还具有抑制肿瘤细胞增殖及诱导细胞衰老与凋亡等作用[5-8]。笔者为了探讨乳腺癌