大叶种普洱茶幼苗对镉胁迫的生理响应
2014-04-29王娅玲李维峰苏志龙罗银铃
王娅玲 李维峰 苏志龙 罗银铃
摘要
[目的]为了研究镉对大叶种普洱茶幼苗叶绿素含量以及过氧化氢酶(CAT)活性、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性以及丙二醛(MDA)含量等生理特性的影响。[方法]以长叶白毫茶苗为供试植物,采用砂培方法。[结果]当镉浓度在0~80 mg/L范围内,茶幼苗叶绿素含量先上升后下降;CAT、POD活性先升高后下降;而SOD活性随着镉浓度的增加含量均逐渐下降,MDA含量则一直呈现增加的趋势。[结论]镉胁迫下大叶种普洱茶幼苗膜系统受到一定程度的破坏。
关键词 镉胁迫;大叶种普洱茶;叶绿素;抗氧化酶
中图分类号 S571 文献标识码 A 文章编号 0517-6611(2014)26-08873-02
Physiolohical Responses of Largeleaf Puer Tea Seedling to Cadmium Stress
WANG Yaling, LUO Yinling et al
(Yunnan Vocational College of Tropical Crops, Puer, Yunnan 665000; Puer University, Puer, Yunnan 665000)
Abstract [Objective] In order to elucidate the influence of cadmium on the largeleaf puer tea seeding chlorophyll content, catalase (CAT) activity, superoxide (SOD) activity, peroxidase (POD) activity and malondialdehyde (MDA) content and their influence on the physiological characteristics. [Method] Changyebaihao tea seeding was adopted in the study using the sand culture method. [Result] The result showed that when cadmium concentration was 0-80 mg/L, chlorophyll content increased first and then decreased. The activities of CAT and POD went up at the beginning of cadmium stress, but they were decreased as the quantity of cadmium increasing. The activities of SOD showed the downtrend as the quantity of cadmium increasing, while the MDA content increased all the time. [Conclusion] The result suggested that the membrane system of largeleaf puer tea seeding was damaged by cadmium stress.
Key words Cadmium stress; Largeleaf puer tea; Chlorophyll; Antioxidant enzyme
重金属镉是最常见的环境污染物之一。过量的镉进入农作物内会对植物的生长造成不利的影响[1],不仅阻碍农作物正常生长发育,而且通过食物链进入人体后对人的神经系统、消化系统、免疫系统等产生危害,严重威胁人类健康[2]。近年来,有关镉对植物的生长及酶活性的影响国内外进行了较多的研究[3-7]。骆耀平等[8]采用盆栽试验对镉胁迫2个茶品种新梢的光合速率及过氧化物酶、超氧化物歧化酶活性进行了研究。王春梅等[9]采用盆栽试验研究了高浓度镉对名山白毫品系茶树生理及吸收积累特性的影响。目前,镉污染对大叶种普洱茶幼苗生理影响的研究未见报道。笔者拟采用砂基水培方法,研究在不同浓度梯度镉胁迫下大叶种普洱茶幼苗叶片叶绿素和抗氧化酶的变化,探讨重金属镉胁迫对大叶种茶幼苗超氧化物歧化酶、过氧化物酶、过氧化氢酶、丙二醛以及叶绿素含量的影响,为大叶种茶幼苗的抗重金属胁迫机制研究提供理论依据,也为正确评估重金属镉胁迫对普洱茶规模化种植造成的损失提供参考。……
