王友利 杨海波 于媛

摘要

[目的] 确定硝酸钠浓度对亚心形扁藻生长及油脂和淀粉含量的影响。[方法] 以亚心形扁藻作为试验材料，以海洋微藻培养常用的普适性康维方营养液为基础，考察硝酸钠质量浓度为10 mg/L、60 mg/L、110 mg/L、160 mg/L和210 mg/L的培养基对亚心形扁藻生长及油脂和淀粉含量的影响。[结果] 当硝酸钠浓度为10 mg/L时，有利于油脂、淀粉的合成，但此时扁藻生长慢，生物量低，使得这些生物组分的总产量低;当硝酸钠质量浓度为60 mg/L时，油脂和淀粉含量较高，并且扁藻生长快，油脂、淀粉的总产量高;继续增加硝酸钠浓度时，扁藻生物量和各组分含量基本不变。[结论] 从微藻生物能源角度考虑，60 mg/L的硝酸钠质量浓度是培养亚心形扁藻的优化浓度。

关键词 亚心形扁藻;硝酸钠;油脂;淀粉;生物量

中图分类号 S968.49  文献标识码 A  文章编号 0517-6611（2014）34-12247-03

Effects of Sodium Nitrate Concentration on the Growth of Platymonas subcordiformis and the Contents of Oil and Starch

WANG Youli， YANG Haibo*， YU Yuan

（Department of Environmental and Chemical Engineering， Dalian University， Dalian， Liaoning 116622）

Abstract [Objective]] To confirm the effects of sodium nitrate concentration on the growth of Platymonas subcordiformis and on the contents of oil and starch in it. [Method] Based on the conventional Kangwei nutrient medium used in culturing marine microalgae， the concentration of sodium nitrate from 10 to 210 mg/L in an interval of 50 mg/L were investigated in increasig the biomass of P. subcordiformis and the contents of oil and starch. [Result] 10 mg/L sodium nitrate was suitable for synthesizing of oil and starch in P. subcordiformis， while P. subcordiformis grew slowly at the concentration because of the lack of nitrogen nutrient. And 60 mg/L sodium nitrate was advantageous to both the biomass of P. subcordiformis and the contents of oil and starch. When the concentration of sodium nitrate was gradually increased， the biomass and the contents changed a few. [Conclusion] From the view of microalgae bioenergy， 60 mg/L sodium nitrate is suitable for culturing P. subcordiformis and extracting oil and starch in it.

Key words  Platymonas subcordiformis; Sodium nitrate; Oil; Starch; Biomass

作者簡介

王友利（1988-），男，山东日照人，硕士研究生，研究方向：海洋微藻生物能源。*通讯作者，教授，从事海洋微藻生物能源研究。

收稿日期 20141027

亚心形扁藻（Platymonas subcordiformis）不仅是水产动物育苗过程中常用的优质饵料，而且因其油脂和淀粉含量高，近些年，逐渐受到微藻生物能源领域研究者的广泛关注[1-2]。

氮是构成海洋微藻生物体的重要元素之一，是微藻生命活动的必需物质，但不同氮源对微藻生长及其细胞内生物组分的合成有着不同影响。刘怀平等[3]的研究表明，在添加NaNO3时，网状空星藻（Coelastrum reticulatum）及栅藻（Scenedesums sp.）的单位体积总脂含量达到最高。Fan等[4]的研究发现以尿素和铵为氮源时，微型原甲藻（Prorocentrum minimum）生长速率增加。……